「20」の検索結果

Build whole-genome alignments using Progressive Cactus (Armstrong 2020 reference-free clade-level WGA), Minigraph-Cactus (Hickey 2024 pangenome-aware), LASTZ chain/net (UCSC pipeline), MUMmer4 (Marçais 2018 pairwise), minimap2 -x asm5/10/20 (Li 2018 fast pairwise), AnchorWave (Song 2022 WGD-aware), and Mauve / progressiveMauve (bacterial). Operates the HAL toolkit (Hickey 2013) for downstream extraction including halSynteny, halLiftover, halBranchMutations, and hal2maf. Use when constructing multi-species alignments for comparative-annotation projection (TOGA), synteny detection, conservation analyses (phyloP / PhastCons), or pangenome graph construction; selecting between reference-free (Cactus) and reference-anchored (LASTZ chains/nets) approaches; tuning sensitivity for closely vs distantly related genomes; or producing HAL files for genome-wide downstream tools.

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Comprehensive methodology for auditing and exploiting TLS/SSL implementations and misconfigurations across network services and mobile applications. Covers protocol downgrade attacks including POODLE (CVE-2014-3566) against SSLv3 CBC padding, DROWN (CVE-2016-0800) cross-protocol attack leveraging SSLv2 export ciphers to decrypt TLS sessions, and FREAK (CVE-2015-0204) forcing RSA export-grade key exchange. Addresses BEAST (CVE-2011-3389) exploiting CBC IV predictability in TLS 1.0, CRIME (CVE-2012-4929) and BREACH targeting TLS-level and HTTP-level compression oracles respectively, and Heartbleed (CVE-2014-0160) for OpenSSL memory disclosure. Covers certificate validation bypass techniques for applications with improper hostname verification or chain validation, certificate pinning bypass using Frida and Objection for mobile application interception, HSTS bypass via NTP manipulation and subdomain exploitation, TLS 1.3 0-RTT replay attacks against non-idempotent endpoints, mutual TLS (mTLS) authentication attacks including client certificate theft and relay, and Certificate Transparency log monitoring for reconnaissance. Primary tooling includes testssl.sh for comprehensive TLS auditing, sslyze for Python-integrated scanning, sslscan for quick cipher enumeration, and tlsx for high-speed TLS probing at scale. Maps to CWE-295 (Improper Certificate Validation), CWE-319 (Cleartext Transmission of Sensitive Information), and CWE-757 (Selection of Less-Secure Algorithm During Negotiation).

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VMware vSphere / vCenter Server external attack matrix — version fingerprinting, the high-impact CVE chain (CVE-2021-21972 vRealize unauth file upload, CVE-2021-21985 vSAN plugin RCE, CVE-2022-22954 Workspace ONE SSTI, CVE-2023-20887 Aria RCE, CVE-2024-37085 ESXi AD bypass, CVE-2023-34048 vCenter DCERPC OOB write APT-exploited), default credentials, SSO configuration disclosure, vmdir LDAP enumeration, ESXi Open SLP RCE history. ONLY for vCenter / Workspace ONE / Aria instances exposed to the internet — internal-network vCenter is out of scope per the external-only boundary. Use when recon shows port 443 with vCenter banner, `/ui` redirect, `/websso/SAML2/Metadata`, or VMware product fingerprints.

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Processes eDNA metabarcoding from raw paired-end reads to species tables, navigating ASV (DADA2, UNOISE3) vs OTU (swarm v2) decision (Callahan 2017 vs Schloss multi-copy-16S critique), marker/primer choice (Leray COI, MiFish 12S, 515F/806R 16S, ITS2) with primer-specific bias, OBITools3 v3 command-name break (obi stats plural; .tar.gz taxonomy), tag-jumping with dual-indexing (Schnell 2015; NovaSeq 10x MiSeq), decontam as screening-not-classifier (Davis 2018), read-counts-not-abundance critique (Lamb 2019), site-occupancy modeling (Ficetola 2015), Naive-Bayes calibration limits (Bokulich 2018), and eDNA decay (Strickler 2015). Use when going from raw eDNA FASTQ to species tables, picking marker + denoising pipeline, deciding whether read counts represent abundance, applying occupancy modeling, configuring OBITools3 v3, or interpreting decontam output. Not for clinical 16S microbiome (see microbiome/amplicon-processing).

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Compute genome-to-genome distances (ANI, AAI, dDDH, k-mer Mash) and assign taxonomic classifications using skani (Shaw 2023), FastANI (Jain 2018), pyani / pyANI ANIb / ANIm, OrthoANI (Lee 2016), AAI (amino-acid identity), dDDH via TYGS / GGDC, GTDB-Tk (Chaumeil 2020 standard prokaryote taxonomy), and Mash MinHash (Ondov 2016). Use when delineating prokaryote species (95% ANI threshold; Jain 2018 Nat Commun 9:5114), assigning genomes to GTDB taxonomy with ANI radius, computing genome similarity matrices for clustering, classifying archaea, evaluating MAG (metagenome-assembled genome) species assignment, applying skani for fast metagenomic ANI screening, or reconciling 16S rRNA-based taxonomy with whole-genome ANI.

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Expert ISO 42001 AI Management System (AIMS) compliance advisor. Use this skill whenever a user asks about ISO/IEC 42001:2023, AI governance, AI management systems, AI risk assessment, AI system impact assessment, Annex A controls for AI, Statement of Applicability for AI systems, AI policy, responsible AI, AI lifecycle management, AI incident management, AI transparency, AI bias, AI certification readiness, or any topic related to implementing or auditing an AI Management System. Also trigger for questions like "how do I become ISO 42001 certified?", "what controls does ISO 42001 require?", "how do I assess AI risk under 42001?", "what is an AIMS?", or any request involving organisational governance of AI systems, responsible AI frameworks, or AI regulatory compliance aligned to an ISO standard.

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Expert ISO 42001 AI Management System (AIMS) compliance advisor. Use this skill whenever a user asks about ISO/IEC 42001:2023, AI governance, AI management systems, AI risk assessment, AI system impact assessment, Annex A controls for AI, Statement of Applicability for AI systems, AI policy, responsible AI, AI lifecycle management, AI incident management, AI transparency, AI bias, AI certification readiness, or any topic related to implementing or auditing an AI Management System. Also trigger for questions like "how do I become ISO 42001 certified?", "what controls does ISO 42001 require?", "how do I assess AI risk under 42001?", "what is an AIMS?", or any request involving organisational governance of AI systems, responsible AI frameworks, or AI regulatory compliance aligned to an ISO standard.

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供应链与生产运营 (供应链与生产运营 (Supply Chain & Production Operations) — 面向制造业与消费品实体供应链的『计划-制造-交付』运营判断,不是采购谈价技巧,也不是跨境电商发货物流。覆盖: (a) 第一性张力 — 这行的核心世界观分歧: **流动 > 局部效率(用有效产出/流动时间看系统,而不是用设备利用率/人均产出看工位;非瓶颈上的效率是幻觉) ⇄ 精益拉动 vs 推式预测(TPS/JIT 按下游消耗拉动、小批量平准化 vs MRP 按预测展开净需求推动;DDMRP 解耦缓冲是第三条路) ⇄ 精益/丰田 TPS 派 vs 约束理论 TOC 派(消除七大浪费、全局改善 vs 五步聚焦瓶颈、鼓-缓冲-绳 DBR 排产;两派互相指责对方浪费时间/只治标) ⇄ 变异是敌人(Deming/六西格玛/Factory Physics 的 VUT 排队论: 利用率越靠近满载排队时间越爆炸,交期长不是产能不够而是变异+高负荷) ⇄ 库存是负债 vs 库存是缓冲(会计把库存记成资产、精益视其为掩盖问题的水位、TOC 与韧性派视其为吸收变异的必要缓冲;真问题是缓冲放在哪一层而不是有没有) ⇄ 精益 JIT vs 韧性冗余(2020-2023 断链后 just-in-case、多源、近岸/友岸的回摆;精益派反驳崩的是『假精益』——单一来源+砍库存不砍变异) ⇄ 服务水平 vs 库存成本(安全库存/报童模型/ABC-XYZ 的经典取舍,牛鞭效应放大上游波动) ⇄ 成本导向压价 vs 供应商共生开发(Kraljic 分级、丰田供应商协会式长期共生 vs 年降指标与竞价) ⇄ 一个数 vs 各部门各算各的(S&OP/IBP 的本质是跨职能对齐到一套数字与一份产销计划))**; (b) 工具栈(此行的工具是『系统+方法』的混合) — ERP/MRP(SAP S/4HANA PP·MM、Oracle、用友·金蝶、Odoo、NetSuite)、APS 高级计划排程(Kinaxis RapidResponse、o9、Blue Yonder、SAP IBP、OMP、Siemens Opcenter APS)、MES/车间执行(Siemens Opcenter、Rockwell FactoryTalk、黑湖智造)、WMS/TMS、需求预测(统计模型 + Nixtla StatsForecast/Prophet、FVA 预测增值分析)、运筹优化与仿真(OR-Tools、Gurobi、Pyomo、AnyLogic、FlexSim、SimPy)、供应链风险与多级可视(Resilinc、Everstream、Interos)、分析层(SQL/Python pandas/Power BI)、以及纸笔工具(价值流图 VSM、A3、看板、标准作业票)—— 选型决策树的关键分岔是 MTS/MTO/ATO/ETO 履行模式与需求变异度,而非厂商功能清单; (c) 心智模型层 — 利特尔法则(在制品 = 产出率 × 流动时间)、瓶颈与非瓶颈、缓冲三态(库存/时间/产能缓冲)、解耦点(客户订单渗透点 CODP)、牛鞭效应、takt time 节拍与产线平衡、OEE 与六大损失、muda·mura·muri 三种浪费、自働化 jidoka 与安灯、防错 poka-yoke、SMED 快速换模换出小批量、平准化 heijunka、PDCA 与丰田改善型 Toyota Kata、DMAIC 与 Cpk 过程能力、报童模型与安全库存公式、ABC-XYZ 分级; (d) 工作流/SOP — 跑一轮月度 S&OP(需求评审→供应评审→预 S&OP→执行 S&OP)、做需求预测与预测误差复盘(MAPE/WMAPE/bias/FVA)、设定库存分级与安全库存、MRP 跑批与例外消息处理(缺料齐套与 MRP 神经质)、瓶颈识别与 DBR 排产、价值流图现状图→未来图→改善计划、新品导入 NPI 与多级 BOM/ECN 工程变更、供应商选择与开发(RFQ→审厂→APQP/PPAP→量产→年度绩效)、断料应急与交期承诺(ATP/CTP)、循环盘点与库存准确率; (e) 知识正典(en/ja 一手极厚,中译齐全) — 《目标 The Goal》Goldratt(TOC 小说体奠基)、《丰田生产方式》大野耐一、《改变世界的机器》Womack·Jones·Roos(MIT IMVP 研究)、《精益思想 Lean Thinking》Womack & Jones、《丰田模式 The Toyota Way》Liker、《工厂物理学 Factory Physics》Hopp & Spearman(排队论视角的运营科学)、《学习观察 Learning to See》Rother & Shook(VSM)、《丰田套路 Toyota Kata》Rother、Spear & Bowen 的 HBR 1999《Decoding the DNA of the Toyota Production System》、Lee/Padmanabhan/Whang 1997《The Bullwhip Effect in Supply Chains》、Ptak & Smith 的 DDMRP、Orlicky 的 MRP、Chopra & Meindl 的供应链管理教材、Deming《转危为安 Out of the Crisis》、Sheffi《The Resilient Enterprise》、ASCM/APICS 的 SCOR 参考模型与 CPIM/CSCP 知识体系; (f) 行业大佬/figures — 大野耐一(TPS 立法者)、新乡重夫(SMED/防错)、Eliyahu Goldratt(TOC 创立者)、W. Edwards Deming(变异与系统观)、James Womack & Daniel Jones(把 TPS 译成西方可学的『精益』)、Jeffrey Liker(丰田模式学术化)、Mike Rother(VSM 与改善型)、Wallace Hopp & Mark Spearman(工厂物理学)、Hau Lee(斯坦福,牛鞭效应与 Triple-A 供应链)、Yossi Sheffi(MIT,韧性)、Carol Ptak & Chad Smith(DDMRP)、David Simchi-Levi(MIT,风险敞口指数)、Steven Spear(TPS 的 DNA)、以及中文圈的供应链实务作者(刘宝红等); (g) 争议/批判(必须保留不洗白) — JIT 是不是 2021 全球断链的元凶(精益派反驳:崩的是单一来源+砍库存不砍变异的『假精益』);西方学精益的高失败率(只搬工具不搬思维,精益在很多工厂沦为裁员的代号);六西格玛黑带官僚化与扼杀创新之争;TOC 派与精益派互相攻击(Goldratt 讽刺精益是等浪费自己浮现);预测无用论(需求驱动派说预测必然错、要靠缓冲吸收)vs 统计预测派;控制塔/数字孪生/AI 预测的过度承诺与高项目失败率;中国制造的年降与内卷式压价 vs 供应商共生;近岸·友岸外包是真实产能转移还是政治叙事; (h) 流派/思想谱系 — **精益/TPS 派(丰田血统:大野-新乡→Womack/Jones/Liker/Rother,信全局消除浪费与人的改善能力)** vs **约束理论 TOC 派(Goldratt:系统产出只由瓶颈决定,聚焦五步、DBR、缓冲管理)** vs **统计质量/六西格玛派(Deming-Juran-Shewhart→GE/Motorola,信减少变异与过程能力)** vs **运营科学/工厂物理学派(Hopp & Spearman:用排队论与数学定律解释,认为精益与 TOC 都是特例)** vs **需求驱动/DDMRP 派(Ptak & Smith:预测不可靠,靠战略解耦缓冲)** vs **韧性/风险派(Sheffi、Simchi-Levi:效率之外必须为断链设计)**。不含: 跨境电商卖家的发货与头程物流(已由 cross-border-ecommerce 覆盖)、纯采购的商务谈判技巧、企业级 ERP 的代码实施与二次开发、通用项目管理。) Master OS — automated mastery of 供应链与生产运营 (Supply Chain & Production Operations) — 面向制造业与消费品实体供应链的『计划-制造-交付』运营判断,不是采购谈价技巧,也不是跨境电商发货物流。覆盖: (a) 第一性张力 — 这行的核心世界观分歧: **流动 > 局部效率(用有效产出/流动时间看系统,而不是用设备利用率/人均产出看工位;非瓶颈上的效率是幻觉) ⇄ 精益拉动 vs 推式预测(TPS/JIT 按下游消耗拉动、小批量平准化 vs MRP 按预测展开净需求推动;DDMRP 解耦缓冲是第三条路) ⇄ 精益/丰田 TPS 派 vs 约束理论 TOC 派(消除七大浪费、全局改善 vs 五步聚焦瓶颈、鼓-缓冲-绳 DBR 排产;两派互相指责对方浪费时间/只治标) ⇄ 变异是敌人(Deming/六西格玛/Factory Physics 的 VUT 排队论: 利用率越靠近满载排队时间越爆炸,交期长不是产能不够而是变异+高负荷) ⇄ 库存是负债 vs 库存是缓冲(会计把库存记成资产、精益视其为掩盖问题的水位、TOC 与韧性派视其为吸收变异的必要缓冲;真问题是缓冲放在哪一层而不是有没有) ⇄ 精益 JIT vs 韧性冗余(2020-2023 断链后

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Processes eDNA metabarcoding from raw paired-end reads to species tables, navigating ASV (DADA2, UNOISE3) vs OTU (swarm v2) decision (Callahan 2017 vs Schloss multi-copy-16S critique), marker/primer choice (Leray COI, MiFish 12S, 515F/806R 16S, ITS2) with primer-specific bias, OBITools3 v3 command-name break (obi stats plural; .tar.gz taxonomy), tag-jumping with dual-indexing (Schnell 2015; NovaSeq 10x MiSeq), decontam as screening-not-classifier (Davis 2018), read-counts-not-abundance critique (Lamb 2019), site-occupancy modeling (Ficetola 2015), Naive-Bayes calibration limits (Bokulich 2018), and eDNA decay (Strickler 2015). Use when going from raw eDNA FASTQ to species tables, picking marker + denoising pipeline, deciding whether read counts represent abundance, applying occupancy modeling, configuring OBITools3 v3, or interpreting decontam output. Not for clinical 16S microbiome (see microbiome/amplicon-processing).

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Compute genome-to-genome distances (ANI, AAI, dDDH, k-mer Mash) and assign taxonomic classifications using skani (Shaw 2023), FastANI (Jain 2018), pyani / pyANI ANIb / ANIm, OrthoANI (Lee 2016), AAI (amino-acid identity), dDDH via TYGS / GGDC, GTDB-Tk (Chaumeil 2020 standard prokaryote taxonomy), and Mash MinHash (Ondov 2016). Use when delineating prokaryote species (95% ANI threshold; Jain 2018 Nat Commun 9:5114), assigning genomes to GTDB taxonomy with ANI radius, computing genome similarity matrices for clustering, classifying archaea, evaluating MAG (metagenome-assembled genome) species assignment, applying skani for fast metagenomic ANI screening, or reconciling 16S rRNA-based taxonomy with whole-genome ANI.

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Knowledge base from the NIST Framework for Cyber-Physical Systems (SP 1500-201/202/203, v1.0, 2017). Use for engineering and analyzing cyber-physical systems (CPS) and IoT: the CPS Framework's aspect × facet grid (nine aspects of concern examined through conceptualization, realization, and assurance facets), cross-property trustworthiness risk management (safety/security/privacy/reliability/resilience together), data interoperability (syntactical/semantic/contextual, canonical models, identifiers, provenance), the timing aspect (time-interval classes, time domains, time-aware networking) and secure/resilient time (GNSS jamming/spoofing, PTP/NTP attacks, holdover, the clock-error model and Allan/MTIE metrics), plus the use-case requirements method. Bridges IT and OT under a clock. Scope limits: a 2017 living/draft document, thin on post-2017 standards and on detailed control catalogs; it names but does not reproduce ISO/IEC/IEEE 42010 and 15288 process text or permission-licensed third-party figures.

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End-to-end eDNA metabarcoding from raw amplicons to community ecology. Covers QC, primer removal (mandatory before DADA2 filterAndTrim), denoising with OBITools3 v3 (obi stats plural; DMS-based) or DADA2 ASVs (Callahan 2017), decontam combined method as screening-not-classifier (Davis 2018), tag-jumping with NovaSeq 10x MiSeq caveat (Schnell 2015), Hill-number effective species counts with coverage-based rarefaction (Jost 2006; Chao & Jost 2012; doubling rule), beta-diversity decomposition with MANDATORY PERMANOVA + PERMDISP pair (Anderson & Walsh 2013), constrained ordination, and the read-counts-not-abundance critique (Lamb 2019). Use when processing eDNA samples for biodiversity assessment, deciding ASV vs OTU, configuring OBITools3 v3, interpreting decontam screening, or reporting community comparisons with the dispersion confound check.

日本語の概要は準備中です。原文の説明を表示しています。

Processes eDNA metabarcoding from raw paired-end reads to species tables, navigating ASV (DADA2, UNOISE3) vs OTU (swarm v2) decision (Callahan 2017 vs Schloss multi-copy-16S critique), marker/primer choice (Leray COI, MiFish 12S, 515F/806R 16S, ITS2) with primer-specific bias, OBITools3 v3 command-name break (obi stats plural; .tar.gz taxonomy), tag-jumping with dual-indexing (Schnell 2015; NovaSeq 10x MiSeq), decontam as screening-not-classifier (Davis 2018), read-counts-not-abundance critique (Lamb 2019), site-occupancy modeling (Ficetola 2015), Naive-Bayes calibration limits (Bokulich 2018), and eDNA decay (Strickler 2015). Use when going from raw eDNA FASTQ to species tables, picking marker + denoising pipeline, deciding whether read counts represent abundance, applying occupancy modeling, configuring OBITools3 v3, or interpreting decontam output. Not for clinical 16S microbiome (see microbiome/amplicon-processing).

日本語の概要は準備中です。原文の説明を表示しています。

Compute genome-to-genome distances (ANI, AAI, dDDH, k-mer Mash) and assign taxonomic classifications using skani (Shaw 2023), FastANI (Jain 2018), pyani / pyANI ANIb / ANIm, OrthoANI (Lee 2016), AAI (amino-acid identity), dDDH via TYGS / GGDC, GTDB-Tk (Chaumeil 2020 standard prokaryote taxonomy), and Mash MinHash (Ondov 2016). Use when delineating prokaryote species (95% ANI threshold; Jain 2018 Nat Commun 9:5114), assigning genomes to GTDB taxonomy with ANI radius, computing genome similarity matrices for clustering, classifying archaea, evaluating MAG (metagenome-assembled genome) species assignment, applying skani for fast metagenomic ANI screening, or reconciling 16S rRNA-based taxonomy with whole-genome ANI.

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Comprehensive methodology for auditing and exploiting TLS/SSL implementations and misconfigurations across network services and mobile applications. Covers protocol downgrade attacks including POODLE (CVE-2014-3566) against SSLv3 CBC padding, DROWN (CVE-2016-0800) cross-protocol attack leveraging SSLv2 export ciphers to decrypt TLS sessions, and FREAK (CVE-2015-0204) forcing RSA export-grade key exchange. Addresses BEAST (CVE-2011-3389) exploiting CBC IV predictability in TLS 1.0, CRIME (CVE-2012-4929) and BREACH targeting TLS-level and HTTP-level compression oracles respectively, and Heartbleed (CVE-2014-0160) for OpenSSL memory disclosure. Covers certificate validation bypass techniques for applications with improper hostname verification or chain validation, certificate pinning bypass using Frida and Objection for mobile application interception, HSTS bypass via NTP manipulation and subdomain exploitation, TLS 1.3 0-RTT replay attacks against non-idempotent endpoints, mutual TLS (mTLS) authentication a...

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VMware vSphere / vCenter Server external attack matrix — version fingerprinting, the high-impact CVE chain (CVE-2021-21972 vRealize unauth file upload, CVE-2021-21985 vSAN plugin RCE, CVE-2022-22954 Workspace ONE SSTI, CVE-2023-20887 Aria RCE, CVE-2024-37085 ESXi AD bypass, CVE-2023-34048 vCenter DCERPC OOB write APT-exploited), default credentials, SSO configuration disclosure, vmdir LDAP enumeration, ESXi Open SLP RCE history. ONLY for vCenter / Workspace ONE / Aria instances exposed to the internet — internal-network vCenter is out of scope per the external-only boundary. Use when recon shows port 443 with vCenter banner, `/ui` redirect, `/websso/SAML2/Metadata`, or VMware product fingerprints.

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External SSL VPN / remote-access appliance attack matrix — Cisco ASA/AnyConnect, Fortinet FortiGate/FortiOS, Citrix NetScaler/ADC, Palo Alto GlobalProtect, Pulse Secure / Ivanti Connect Secure, SonicWall, F5 Big-IP. Covers version fingerprinting, CVE matrix (2018-2026), AAA backend identification, default credentials, configuration-disclosure paths, pre-auth RCE/SSRF/path-traversal exploits where applicable. Built from authorized-engagement Cisco ASA testing plus 2024-2026 enterprise VPN CVE landscape. Use whenever the target's perimeter exposes any SSL VPN appliance or remote-access gateway — these are the most common initial-access points in 2024-2026 actor TTPs.

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Guide users through writing a systematic literature review (SLR) following the PRISMA 2020 framework. Use this skill whenever the user mentions 'systematic review', 'systematic literature review', 'SLR', 'PRISMA', 'PRISMA 2020', 'PRISMA flow diagram', 'PRISMA checklist', or asks for help writing, structuring, or auditing a literature review that follows reporting guidelines. Also trigger when the user asks about inclusion/exclusion criteria for a review, search strategies for databases like Scopus/WoS/PubMed, study selection processes, risk of bias assessment, or narrative synthesis for a review paper. This skill covers the full PRISMA 2020 checklist (27 items), produces a Word document manuscript in strict journal article format, generates an annotated PRISMA flow diagram, and enforces APA 7th Edition referencing throughout. It does NOT cover meta-analysis or statistical pooling. By Chuah Kee Man.

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Build and maintain a Stockbee-style daily 20% mover study for US equities by scanning +20%/-20% movers, classifying catalysts and setup context, updating forward outcomes, and summarizing cohort patterns. Use when the user asks to run a daily 20% study, backfill historical 20% movers, find recurring edge patterns, or build a model book of explosive market moves.

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Designs and analyzes combinatorial CRISPR screens covering paired-Cas9 (Big Papi, Najm 2018), enhanced AsCas12a multiplex (enCas12a, DeWeirdt 2021), in4mer 4-guide-array Cas12a (Esmaeili Anvar N et al 2024 Nat Commun 15:3577) and the Inzolia paralog-pair library, paralog-buffering detection (Dede 2020 Genome Biol; Thompson 2021 Nat Commun 12:1302), genetic-interaction (GI) scoring as observed_double_LFC minus expected_additive_double_LFC, synthetic-lethal and synthetic-rescue interaction interpretation, the half-of-essentiality buffered by paralogs phenomenon, multiplex screen statistical analysis with MAGeCK MLE interaction terms, and the relationship to single-cell combinatorial Perturb-seq. Use when designing a paralog or pathway-pair screen, choosing between paired-Cas9 (Big Papi) and Cas12a multiplex (Inzolia), interpreting genetic interaction scores, identifying synthetic-lethal targets for drug development, or scaling beyond single-gene CRISPR screens.

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Detect, date, and contextualize whole-genome duplication (WGD / paleopolyploidy) events using wgd v2 (Chen et al 2024), KsRates (Sensalari 2022 substitution-rate-corrected Ks dating), DupGen_finder (Qiao 2019), MAPS (Li 2018 phylogenomic), POInT (Conant 2008 ordered-block), SLEDGe (2024 ML-based), Whale.jl (Bayesian DL+WGD), and synteny-anchored paranome construction. Use when identifying ancient polyploidy from Ks distributions and synteny block analysis, positioning WGD events relative to speciation, distinguishing tandem from segmental from WGD duplications, dating the 2R/3R vertebrate / fish / salmonid WGDs, building paranome and Ks-age mixture models, applying KsRates substitution-rate correction across lineages, or testing alternative biased-fractionation / dosage-balance models post-WGD.

日本語の概要は準備中です。原文の説明を表示しています。

Designs and analyzes combinatorial CRISPR screens covering paired-Cas9 (Big Papi, Najm 2018), enhanced AsCas12a multiplex (enCas12a, DeWeirdt 2021), in4mer 4-guide-array Cas12a (Esmaeili Anvar N et al 2024 Nat Commun 15:3577) and the Inzolia paralog-pair library, paralog-buffering detection (Dede 2020 Genome Biol; Thompson 2021 Cell Reports 36:109597), genetic-interaction (GI) scoring as observed_double_LFC minus expected_additive_double_LFC, synthetic-lethal and synthetic-rescue interaction interpretation, the half-of-essentiality buffered by paralogs phenomenon, multiplex screen statistical analysis with MAGeCK MLE interaction terms, and the relationship to single-cell combinatorial Perturb-seq. Use when designing a paralog or pathway-pair screen, choosing between paired-Cas9 (Big Papi) and Cas12a multiplex (Inzolia), interpreting genetic interaction scores, identifying synthetic-lethal targets for drug development, or scaling beyond single-gene CRISPR screens.

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Project gene annotations across genomes using TOGA (Kirilenko 2023 whole-genome-alignment chain-based projection with intactness classification), CESAR 2.0 (Sharma & Hiller 2017 codon-aware exon projection), LiftOff (Shumate & Salzberg 2020 reference-based annotation transfer), Liftover (UCSC), GeMoMa (Keilwagen 2019 evidence-based projection), and Comparative Annotation Toolkit (CAT). Use when transferring annotations from a well-annotated reference to query genome(s), classifying gene-loss vs gene-intact across many genomes at scale, building Zoonomia-style comparative annotations across hundreds of mammals or birds (Kirilenko 2023), detecting pseudogenization, projecting alternative isoforms, or selecting between WGA-anchored (TOGA) vs ortholog-based (LiftOff) annotation transfer strategies.

日本語の概要は準備中です。原文の説明を表示しています。

Web 线上展览开发 (虚拟展厅) (Web 线上展览 / 虚拟展厅开发 (从业者/工程视角) — 用 web 技术开发线上展览、虚拟展厅、云展会、数字展馆，覆盖 3D 空间、全景漫游、WebXR、文博数字化、营销云展厅、3D 商品展示等形态。覆盖: (a) 技术栈 — 3D web(Three.js / React Three Fiber / Babylon.js / Google model-viewer / WebGL / WebGPU)、全景(Pannellum / Marzipano / Photo Sphere Viewer / krpano / 720云)、WebXR(WebXR Device API / A-Frame)、资产管线(glTF·GLB / Draco / Meshopt / KTX2 纹理压缩 / gltf-transform / LOD); (b) 自研 vs SaaS 平台选型(国内: 众趣 / 酷雷曼 / 比目鱼 / 会鸽 / 3DVista; 海外: Matterport / Kuula / Artsteps / Spatial); (c) 性能优化(大场景加载 / 模型轻量化 / draw call / 移动端 / 首屏 / 显存)——web 3D 第一性难题; (d) 策展与导览(动线设计 / 热点 hotspot / 语音讲解 / 交互); (e) 文博数字化(IIIF / 数字孪生 / 高清文物) 与 会展·电商商业化(营销云展厅 / 数据埋点 / 线索转化); (f) 部署(CDN / 流式加载 / 跨端兼容 / WebGL 降级)。学派分歧: 自研 Three.js vs SaaS 平台、真 3D 重场景 vs 720 全景轻量、WebGL vs WebGPU、文博考据派 vs 营销转化派、沉浸优先 vs 加载优先。不含: 线下展陈 / 展台搭建、原生 VR App(非 web)、通用 3D 游戏开发、纯 3D 建模教程。) Master OS — automated mastery of Web 线上展览 / 虚拟展厅开发 (从业者/工程视角) — 用 web 技术开发线上展览、虚拟展厅、云展会、数字展馆，覆盖 3D 空间、全景漫游、WebXR、文博数字化、营销云展厅、3D 商品展示等形态。覆盖: (a) 技术栈 — 3D web(Three.js / React Three Fiber / Babylon.js / Google model-viewer / WebGL / WebGPU)、全景(Pannellum / Marzipano / Photo Sphere Viewer / krpano / 720云)、WebXR(WebXR Device API / A-Frame)、资产管线(glTF·GLB / Draco / Meshopt / KTX2 纹理压缩 / gltf-transform / LOD); (b) 自研 vs SaaS 平台选型(国内: 众趣 / 酷雷曼 / 比目鱼 / 会鸽 / 3DVista; 海外: Matterport / Kuula / Artsteps / Spatial); (c) 性能优化(大场景加载 / 模型轻量化 / draw call / 移动端 / 首屏 / 显存)——web 3D 第一性难题; (d) 策展与导览(动线设计 / 热点 hotspot / 语音讲解 / 交互); (e) 文博数字化(IIIF / 数字孪生 / 高清文物) 与 会展·电商商业化(营销云展厅 / 数据埋点 / 线索转化); (f) 部署(CDN / 流式加载 / 跨端兼容 / WebGL 降级)。学派分歧: 自研 Three.js vs SaaS 平台、真 3D 重场景 vs 720 全景轻量、WebGL vs WebGPU、文博考据派 vs 营销转化派、沉浸优先 vs 加载优先。不含: 线下展陈 / 展台搭建、原生 VR App(非 web)、通用 3D 游戏开发、纯 3D 建模教程。: top builders' mental models, tool stack, current workflows, jargon, and where to keep up. Trigger this skill when the user works on Web 线上展览 / 虚拟展厅开发 (从业者/工程视角) — 用 web 技术开发线上展览、虚拟展厅、云展会、数字展馆，覆盖 3D 空间、全景漫游、WebXR、文博数字化、营销云展厅、3D 商品展示等形态。覆盖: (a) 技术栈 — 3D web(Three.js / React Three Fiber / Babylon.js / Google model-viewer / WebGL / WebGPU)、全景(Pannellum / Marzipano / Photo Sphere Viewer / krpano / 720云)、WebXR(WebXR Device API / A-Frame)、资产管线(glTF·GLB / Draco / Meshopt / KTX2 纹理压缩 / gltf-transform / LOD); (b) 自研 vs SaaS 平台选型(国内: 众趣 / 酷雷曼 / 比目鱼 / 会鸽 / 3DVista; 海外: Matterport / Kuula / Artsteps / Spatial); (c) 性能优化(大场景加载 / 模型轻量化 / draw call / 移动端 / 首屏 / 显存)——web 3D 第一性难题; (d) 策展与导览(动线设计 / 热点 hotspot / 语音讲解 / 交互); (e) 文博数字化(IIIF / 数字孪生 / 高清文物) 与 会展·电商商业化(营销云展厅 / 数据埋点 / 线索转化); (f) 部署(CDN / 流式加载 / 跨端兼容 / WebGL 降级)。学派分歧: 自研 Three.js vs SaaS 平台、真 3D 重场景 vs 720 全景轻量、WebGL vs WebGPU、文博考据派 vs 营销转化派、沉浸优先 vs 加载优先。不含: 线下展陈 / 展台搭建、原生 VR App(非 web)、通用 3D 游戏开发、纯 3D 建模教程。 problems and wants industry-grade thinking, tool selection, or workflow guidance. 触发词：「线上展览」「虚拟展厅」「web 线上展览」「云展厅」「数字展馆」

日本語の概要は準備中です。原文の説明を表示しています。