38 件 ・ 関連度順
概要と使いどころ
Access NIH Metabolomics Workbench via REST API (4,200+ studies). Query metabolites, RefMet nomenclature, MS/NMR data, m/z searches, study metadata, for metabolomics and biomarker discovery.
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GATK Best Practices for germline SNP/indel calling from WGS/WES BAMs. Per-sample HaplotypeCaller GVCFs, GenomicsDBImport, GenotypeGVCFs joint calling, VQSR or hard filters. Requires BWA-MEM2-aligned, markdup, BQSR BAMs. Use DeepVariant for a faster DL alternative; GATK is the NIH/ENCODE standard.
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Query Metabolomics Workbench REST API (4,200+ NIH studies) for metabolite ID, study discovery, RefMet standardization, m/z precursor searches, and gene/protein annotations. Quirks: compound input_item rejects `name` (use pubchem_cid/kegg_id/inchi_key/etc.); free-text → compound is a two-step refmet/match→refmet/name flow; moverz endpoint returns TSV text, not JSON. Use hmdb-database for local XML; pubchem-compound-search for general compound lookup.
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Think and work like an expert Emergency Medicine Researcher. Use when a task calls for Emergency Medicine Researcher judgment. ED trial design expert for pragmatic and cluster RCTs, time-zero and immortal-time bias in acute cohorts, NIHSS/SOFA/qSOFA and ESI/CTAS triage, NEDS/NHAMCS registries, and CONSORT/SPIRIT reporting with Hawthorne and selection-bias failure modes.
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Run 150+ AI apps via inference.sh CLI - image generation, video creation, LLMs, search, 3D, Twitter automation. Models: FLUX, Veo, Gemini, Grok, Claude, Seedance, OmniHuman, Tavily, Exa, OpenRouter, and many more. Use when running AI apps, generating images/videos, calling LLMs, web search, or automating Twitter. Triggers: inference.sh, infsh, ai model, run ai, serverless ai, ai api, flux, veo, claude api, image generation, video generation, openrouter, tavily, exa search, twitter api, grok
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Use when a task needs the judgment of a Biology Professor at a research university — writing or defending an NIH/NSF grant budget, deciding authorship order on a paper, navigating an IACUC or biosafety protocol, mentoring a PhD student through a stalled project, or converting a course when active-learning data doesn't match student pushback.
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Run 150+ AI applications in the cloud via the inference.sh platform. Triggers on "generate image with FLUX", "create video", "use Veo/Seedance", "run inference.sh", "infsh CLI", "single key for image+video+LLM". One API key covers image generation (FLUX, Reve, Seedream, Grok, Gemini), video (Veo, Wan, Seedance, OmniHuman, HunyuanVideo), LLMs (Claude, Gemini, Kimi, GLM-4), search (Tavily, Exa), 3D (Rodin), social (Twitter/X), and audio (TTS, voice cloning).
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Europe PMC RESTful Web Service 多源文献聚合检索原子 skill。**使用本 skill 进行医学文献广召回 / RAG 知识库构建 / 跨库聚合 / 预印本扫描 / 全文链接发现**。一次调用同时召回 PubMed (MED) + PMC + AGRICOLA + CABI + 预印本(bioRxiv / medRxiv / ChemRxiv / Research Square)+ NCBI Bookshelf + 专利,自动按 PMID/PMCID/DOI 三级去重,返回完整元数据(全文 OA 链接、citations、grants 资助信息)。**定位:广召回 · 多源 · RAG 友好**(与 pubmed-eutils 互补 — 后者做单源精准官方检索 + Clinical Queries;本 skill 做"撒网"式多源聚合)。触发词:Europe PMC / EuropePMC / 多源文献聚合 / 广召回 / RAG 召回 / RAG 知识库 / 预印本搜索 / preprint search / bioRxiv / medRxiv / ChemRxiv / DOI 检索 / DOI lookup / 全文链接 / full-text links / OA 全文 / PMCID / 灰色文献 / grey literature / 跨库去重 / 资助检索 / grant search / NIH funded / Wellcome funded / citations metadata。**不做**:NCBI E-utilities 直检(→ pubmed-eutils)、临床试验注册(→ clinical-trials-v2)、全文 XML 抽取(→ bioc-fulltext-fetch)、实体识别(→ pubtator-entity-search)、证据分级(→ medical-evidence-grading)、参考文献终态写入(→ evidence-appendix-sync)。
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Generate AI videos with Google Veo, Seedance, Wan, Grok and 40+ models via inference.sh CLI. Models: Veo 3.1, Veo 3, Seedance 1.5 Pro, Wan 2.5, Grok Imagine Video, OmniHuman, Fabric, HunyuanVideo. Capabilities: text-to-video, image-to-video, lipsync, avatar animation, video upscaling, fole...
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Run 150+ AI apps via inference.sh CLI - image generation, video creation, LLMs, search, 3D, Twitter automation. Models: FLUX, Veo, Gemini, Grok, Claude, Seedance, OmniHuman, Tavily, Exa, OpenRouter, and many more. Use when running AI apps, generating images/videos, calling LLMs, web search...
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Build multi-step AI content creation pipelines combining image, video, audio, and text. Workflow examples: generate image -> animate -> add voiceover -> merge with music. Tools: FLUX, Veo, Kokoro TTS, OmniHuman, media merger, upscaling. Use for: YouTube videos, social media content, market...
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Reads, queries, and writes bigWig indexed binary signal tracks (coverage, fold-change, conservation, methylation-rate) with pyBigWig (Python) and the UCSC Kent tools (bedGraphToBigWig, bigWigToBedGraph, bigWigInfo, bigWigSummary, bigWigAverageOverBed) and deepTools (multiBigwigSummary, computeMatrix, bigwigCompare). Covers the central trap that a wide query returns a precomputed zoom-level summary (by default the mean, which annihilates narrow peaks) not per-base data, when exact=True/values() is mandatory, the NaN-not-zero gap-handling fork, choosing mean vs max vs sum vs coverage by biological question, and the sorted-bedGraph plus chrom.sizes build requirement. Use when extracting signal at regions, computing mean signal per gene/peak, building a browser track from bedGraph, comparing tracks, or building TSS/gene-body metaprofiles.
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Detects aberrant splicing in single rare-disease patients vs a control panel using FRASER 2.0 (Bioconductor; Beta-binomial autoencoder on Intron Jaccard Index, default delta cutoff 0.1, q hyperparameter), OUTRIDER (gene-level outlier expression via autoencoder denoising), LeafcutterMD (Dirichlet-multinomial outlier mode of LeafCutter for annotation-free junctions), and DROP (Snakemake pipeline integrating FRASER2 + OUTRIDER + monoallelic expression for clinical diagnostics). The statistical model is fundamentally different from differential splicing — single-sample-vs-cohort outlier detection rather than two-group comparison. Standard tool in EU rare-disease (Solve-RD) and NIH UDN programs. Use when applying RNA-seq to undiagnosed Mendelian disease, validating predicted splice variants in clinical samples, or detecting cryptic splicing in disease tissue.
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Write competitive research proposals for NSF, NIH, DOE, and DARPA. Agency-specific formatting, review criteria, budget preparation, broader impacts, significance statements, innovation narratives, and compliance with submission requirements.
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