「assembly」の検索結果

Reverse engineers malware binaries using NSA's Ghidra disassembler and decompiler to understand internal logic, cryptographic routines, C2 protocols, and evasion techniques at the assembly and pseudo-C level. Activates for requests involving malware reverse engineering, disassembly analysis, decompilation, binary analysis, or understanding malware internals.

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End-to-end shotgun metagenomics workflow from FASTQ to taxonomic and functional profiles, orchestrating controls/host depletion, Kraken2+Bracken classification, MetaPhlAn marker profiling, and HUMAnN functional profiling. Covers the controls-first ordering, why Kraken2 read counts are not abundances and MetaPhlAn cell fractions do not equal Bracken read fractions, and the consistent-pipeline framing. Use when profiling shotgun metagenomic samples end to end, or chaining classification, abundance, and function. For resistome see metagenomics/amr-detection; for strains see metagenomics/strain-tracking; for assembly see genome-assembly/metagenome-assembly.

日本語の概要は準備中です。原文の説明を表示しています。

Assembles microbial-community sequencing into metagenome-assembled genomes (MAGs) with metaFlye (ONT), metaSPAdes/MEGAHIT (Illumina), and hifiasm-meta/metaMDBG (PacBio HiFi), then recovers genomes via multi-binner consolidation (MetaBAT2, MaxBin2, CONCOCT, SemiBin2, VAMB -> DAS_Tool) and QCs them against MIMAG with CheckM2, GUNC, and GTDB-Tk. Covers why a metagenome is not a genome (uneven coverage, micro-diversity, strain collapse to consensus), differential-coverage binning, co-assembly vs per-sample, the rRNA-operon collapse that fails short-read MAGs, and strain resolution with inStrain. Use when reconstructing genomes from a microbiome, soil, ocean, or gut community, recovering MAGs, or resolving strain-level variation.

日本語の概要は準備中です。原文の説明を表示しています。

Detects and removes contamination in genome assemblies via two disjoint workflows - foreign-sequence screening of a single-organism (eukaryote/isolate) assembly with NCBI FCS-GX (GenBank-submission-mandatory), FCS-adaptor, and BlobToolKit blob plots; and MAG/bin quality assessment with CheckM2 plus GUNC (chimerism) plus GTDB-Tk taxonomy, judged against MIMAG. Covers why CheckM2 alone is blind to disjoint-marker chimeras, the FCS-GX RAM wall, organelle/NUMT triage, strain heterogeneity, and the HGT-vs-contamination (tardigrade) trap. Use when screening an assembly for foreign contamination before GenBank submission, assessing MAG completeness/contamination/chimerism, deciding which contigs to remove, or distinguishing real HGT from contaminant contigs.

日本語の概要は準備中です。原文の説明を表示しています。

Assembles a genome de novo from Illumina short reads with SPAdes (isolate/careful/sc/meta/plasmid/rna modes), MEGAHIT (low-memory, huge datasets), Unicycler (bacterial finishing/hybrid), MaSuRCA (large hybrid), ABySS (Bloom-filter), and Platanus (heterozygous diploids), using multi-k de Bruijn graphs. Covers the repeat-resolution limit, why N50 plateaus at the genome not the depth, GenomeScope2 k-mer profiling first, the heterozygosity/haplotig trap, error-correction erasing rare alleles, GC dropout, and NG50/auN/BUSCO reporting. Use when assembling a bacterial isolate, fungal, small-eukaryotic, single-cell, or metagenome genome from Illumina reads, or when deciding whether short reads can even produce the assembly being asked for.

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Assembles haplotype-resolved diploid and telomere-to-telomere (T2T) genomes from PacBio HiFi reads with hifiasm (HiFi-only, Hi-C, or trio phasing) and verkko (HiFi + ultralong ONT for T2T), extracting contigs from GFA and routing phasing QC to k-mer/trio metrics. Covers why a primary assembly is a haplotype mosaic that exists in no cell, partial-vs-full phasing (the .bp. vs .dip. filename convention), the purge-default trap on inbred samples, the --hom-cov coverage-estimate alarm, and verkko-vs-hifiasm for T2T. Use when assembling a diploid eukaryote from HiFi, phasing haplotypes with parents (trio) or Hi-C, deciding whether to chase T2T, or diagnosing switch errors invisible to N50/BUSCO/QV.

日本語の概要は準備中です。原文の説明を表示しています。

Select, document, download, and checksum-verify the most appropriate NCBI reference genome on an HPC cluster. Use whenever an analysis needs a reference genome, genome FASTA, genome annotation, assembly accession, or genome index and no exact approved assembly is already available; when a user asks to fetch a species genome from NCBI; or when a pipeline must resolve and cache a reproducible reference assembly on a remote cluster.

日本語の概要は準備中です。原文の説明を表示しています。

Converts genomic intervals between coordinate conventions, normalises and compares variant representations, and detects assembly or contig-naming mismatches before they corrupt an analysis. Used whenever coordinates cross a format, tool, or assembly boundary - converting between BED, GFF/GTF, VCF, SAM/BAM, WIG, PSL, genePred, Picard interval_list, or region strings; reconciling 0-based half-open with 1-based inclusive; left-aligning or trimming indels; checking whether two variant records describe the same change; mapping genomic to transcript, CDS, or protein positions; auditing a BED/GTF/VCF for convention violations; or diagnosing GRCh37 vs hg19 vs GRCh38 vs T2T, chr-prefix, and liftover problems. Triggers include "off by one", "0-based", "1-based", "half-open", "coordinate system", "left-align", "normalize variant", "bcftools norm", "chr prefix", "wrong genome build", "liftover", "REF mismatch", and "HGVS".

日本語の概要は準備中です。原文の説明を表示しています。

.NET / C# 二进制逆向。当目标是 .NET assembly（PE 头含 CLR、.exe/.dll 托管程序）、C# 编译产物（含 NativeAOT）、红队 Sharp* 工具（Rubeus / SharpHound / SharpHound 等）、.NET 混淆程序（ConfuserEx / SmartAssembly / Babel / Eazfuscator）、.NET loader / info-stealer / 套壳 malware 时使用。优先用 dnSpyEx + de4dot，需要 AI 直接操作时联动 dnSpy MCP。不用于纯 native 二进制（走 reverse-engineering / ida-reverse）。

日本語の概要は準備中です。原文の説明を表示しています。

Guides conversion of a pre-.NET 8 Blazor Server app into a .NET 8+ Blazor Web App. USE FOR: migrating apps that use AddServerSideBlazor and MapBlazorHub to the AddRazorComponents/MapRazorComponents model, converting _Host.cshtml to an App.razor root component, replacing blazor.server.js with blazor.web.js, migrating CascadingAuthenticationState to a service, adopting new Blazor Web App features like enhanced navigation and streaming rendering. DO NOT USE FOR: apps that are already Blazor Web Apps (already use AddRazorComponents and MapRazorComponents), Blazor WebAssembly or hosted Blazor WebAssembly apps (different migration path), apps that should stay on the Blazor Server hosting model without converting, or apps still targeting .NET Framework.

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The reference agents' cache-stable request assembly, covering the static system and per-request context split, the fixed tool list, the rolling conversation breakpoint, which config fields are prompt bytes, and verification. Load when writing or reviewing a commerce agent's prompt assembly, when cache reads are zero, or when a turn's latency or cost is the question.

日本語の概要は準備中です。原文の説明を表示しています。

JLCPCB PCB fabrication and assembly — BOM/CPL generation, basic vs extended parts, assembly constraints, design rules, ordering workflow. Use with KiCad for JLCPCB manufacturing. Use this skill when the user mentions JLCPCB, wants to order PCBs or assembled boards, needs prototype bare PCBs and stencils, wants to know JLCPCB design rules and capabilities, or is asking about PCB manufacturing costs or turnaround times. For gerber/CPL export, stencil ordering, and BOM management, see the `bom` skill.

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Search LCSC Electronics for electronic components — find parts by LCSC number (Cxxxxx) or MPN, check stock/pricing, download datasheets, analyze specifications. Sister company to JLCPCB, same parts library. Sync and maintain a local datasheets directory for a KiCad project, or use batch MPN-list seeding (`--mpn-list`) for bulk workflows without a project. No API key needed — uses the free jlcsearch community API. Use this skill when the user mentions LCSC, JLCPCB parts library, JLCPCB assembly parts, production sourcing, Cxxxxx part numbers, needs to find LCSC equivalents for parts, is preparing a BOM for JLCPCB assembly, or wants to download datasheets and LCSC is available. For package cross-reference tables and BOM workflow, see the `bom` skill.

日本語の概要は準備中です。原文の説明を表示しています。

Search LCSC Electronics for electronic components — find parts by LCSC number (Cxxxxx) or MPN, check stock/pricing, download datasheets, analyze specifications. Sister company to JLCPCB, same parts library. Sync and maintain a local datasheets directory for a KiCad project, or use batch MPN-list seeding (`--mpn-list`) for bulk workflows without a project. No API key needed — uses the free jlcsearch community API. Use this skill when the user mentions LCSC, JLCPCB parts library, JLCPCB assembly parts, production sourcing, Cxxxxx part numbers, needs to find LCSC equivalents for parts, is preparing a BOM for JLCPCB assembly, or wants to download datasheets and LCSC is available. For package cross-reference tables and BOM workflow, see the `bom` skill.

日本語の概要は準備中です。原文の説明を表示しています。

JLCPCB PCB fabrication and assembly — BOM/CPL generation, basic vs extended parts, assembly constraints, design rules, ordering workflow. Use with KiCad for JLCPCB manufacturing. Use this skill when the user mentions JLCPCB, wants to order PCBs or assembled boards, needs prototype bare PCBs and stencils, wants to know JLCPCB design rules and capabilities, or is asking about PCB manufacturing costs or turnaround times. For gerber/CPL export, stencil ordering, and BOM management, see the `bom` skill.

日本語の概要は準備中です。原文の説明を表示しています。

Assemble a creator picture-in-picture product-listicle ad from a config — the creator stays FULL-FRAME the whole beat (voice plus lips generated together per beat, no separate VO, no cut to a full-frame product shot), and on each product beat three persistent overlays ride on top for the WHOLE beat — a title pill top-center, the DEMO in a rounded PiP window top-right (the brand's real UGC clip MUTED, or for a no-UGC brand the product's own autocropped UI still / screen-recording sized to fill the window), and a bottom product card (rounded thumbnail plus 'N · CATEGORY' small-caps plus product NAME in a serif face). Hook plus CTA beats are the creator full-frame with the title pill only. Assembly builds ONE full-1080x1920 transparent overlay PNG per beat, overlays it on the creator clip (cover-scaled to 1080x1920) for the whole beat keeping the native audio, concats all beats, then burns captions LAST as timed PIL PNG overlays (this ffmpeg has no libass) timed deterministically from the known per-beat script. This is the FREE deterministic assembly stage (overlay-PNG build plus cover-scale composite plus concat plus PIL-PNG caption burn); the creator anchor and the N native talking clips come from create-image-fal (Seedream v5 Pro) and create-video-fal (Seedance 2.0). Use for the creator-pip-listicle format.

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Orders and orients assembled contigs into chromosome-scale scaffolds from long-range linking data, inserting N-gap spacers (adds no sequence). Covers Hi-C/Omni-C scaffolding (YaHS, SALSA2, 3D-DNA/Juicer), Hi-C read-mapping prerequisites (map each end separately, no mate rescue, dedup, enzyme-aware), reading the contact map for misjoins/inversions/false-duplications, manual curation in Juicebox/PretextView (the VGP/DToL standard), reference-guided scaffolding (RagTag) and its karyotype-erasure hazard, genetic-map (ALLMAPS) and Bionano optical-map integration, chimera-breaking before scaffolding, gap-filling, and telomere/contig-vs-scaffold-N50 QC (tidk). Use when turning contigs into chromosomes with Hi-C, integrating a linkage map or optical map, choosing a scaffolder by available linking data, or judging whether a chromosome-scale assembly is trustworthy.

日本語の概要は準備中です。原文の説明を表示しています。

.NET / C# 二进制逆向。当目标是 .NET assembly（PE 头含 CLR、.exe/.dll 托管程序）、C# 编译产物（含 NativeAOT）、红队 Sharp* 工具（Rubeus / SharpHound / SharpHound 等）、.NET 混淆程序（ConfuserEx / SmartAssembly / Babel / Eazfuscator）、.NET loader / info-stealer / 套壳 malware 时使用。优先用 dnSpyEx + de4dot，需要 AI 直接操作时联动 dnSpy MCP。不用于纯 native 二进制（走 reverse-engineering / ida-reverse）。

日本語の概要は準備中です。原文の説明を表示しています。

Guides conversion of a pre-.NET 8 Blazor Server app into a .NET 8+ Blazor Web App. USE FOR: migrating apps that use AddServerSideBlazor and MapBlazorHub to the AddRazorComponents/MapRazorComponents model, converting _Host.cshtml to an App.razor root component, replacing blazor.server.js with blazor.web.js, migrating CascadingAuthenticationState to a service, adopting new Blazor Web App features like enhanced navigation and streaming rendering. DO NOT USE FOR: apps that are already Blazor Web Apps (already use AddRazorComponents and MapRazorComponents), Blazor WebAssembly or hosted Blazor WebAssembly apps (different migration path), apps that should stay on the Blazor Server hosting model without converting, or apps still targeting .NET Framework.

日本語の概要は準備中です。原文の説明を表示しています。

Build and review Blazor applications across server, WebAssembly, web app, and hybrid scenarios with correct component design, state flow, rendering, and hosting choices. USE FOR: building interactive web UIs with C# instead of JavaScript; choosing between Server, WebAssembly, or Auto render modes; designing component hierarchies and state. DO NOT USE FOR: unrelated stacks; generic tasks that do not need this specific guidance. INVOKES: inspect the repository context, edit targeted files, and run relevant build, test, lint, or validation commands when changes are made.

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Build cross-platform .NET applications with Uno Platform targeting WebAssembly, iOS, Android, macOS, Linux, and Windows from a single XAML/C# codebase. USE FOR: building cross-platform apps from a single C# and XAML codebase; targeting WebAssembly, iOS, Android, macOS, Linux, and Windows simultaneously; migrating WPF or UWP. DO NOT USE FOR: unrelated stacks; generic tasks that do not need this specific guidance. INVOKES: inspect the repository context, edit targeted files, and run relevant build, test, lint, or validation commands when changes are made.

日本語の概要は準備中です。原文の説明を表示しています。

Large scene layout in Blender with collections, linking, overrides, streaming-friendly organization, and set assembly via MCP.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when an agent needs AssemblyAI for speech-to-text or speech-understanding work in media production, including pre-recorded, synchronous short-file, and real-time streaming transcription; speaker diarization or speaker identification; captions and subtitles; timestamps; language detection, code-switching, or transcript translation; audio intelligence such as summaries, chapters, topics, entities, key phrases, sentiment, content moderation, profanity filtering, and PII redaction; webhooks, scaling, rate limits, retention, security, consent, and QA for podcasts, interviews, captions, call recordings, and edit workflows.

日本語の概要は準備中です。原文の説明を表示しています。

.NET / C# 二进制逆向。当目标是 .NET assembly（PE 头含 CLR、.exe/.dll 托管程序）、C# 编译产物（含 NativeAOT）、红队 Sharp* 工具（Rubeus / SharpHound / SharpHound 等）、.NET 混淆程序（ConfuserEx / SmartAssembly / Babel / Eazfuscator）、.NET loader / info-stealer / 套壳 malware 时使用。优先用 dnSpyEx + de4dot，需要 AI 直接操作时联动 dnSpy MCP。不用于纯 native 二进制（走 reverse-engineering / ida-reverse）。

日本語の概要は準備中です。原文の説明を表示しています。