633 件 ・ 関連度順
概要と使いどころ
Provides efficient containers for storing and manipulating short genomic alignments (typically obtained by aligning short reads to a reference genome). This includes read counting, computing the coverage, junction detection, and working wit
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Build brand partnership strategy — co-branding campaigns, brand collaborations, licensing deals, partner brand alignment, and joint marketing. Use when the user says "brand partnership", "brand collab", "co-branding", "brand collaboration", "partnership marketing", "brand alliance", "co-branded product", "licensing deal", "partner with another brand", "brand collaboration strategy", "how do we partner with brands", "co-marketing partnership", "joint campaign", or wants to build credibility, reach, or revenue through alignment with another brand.
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Splice-aware RNA-seq aligner producing sorted BAM and splice junction tables. Builds genome index, runs two-pass alignment for better junctions. Outputs sorted BAM, junctions (SJ.out.tab), stats (Log.final.out), optional gene counts. Use Salmon for fast pseudoalignment; STAR when a BAM is needed for variant calling, IGV, or ENCODE pipelines.
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CLI toolkit for SAM/BAM/CRAM: sort, index, convert, filter, QC alignments. Core commands: view, sort, index, flagstat, stats, depth, markdup, merge. Required between alignment and variant/peak calling. Use pysam for Python-native BAM access; deeptools for normalized coverage tracks.
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Routes face-alignment installation, import checks, landmark-detection workflows, and detector-backend selection.
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Quantifies CRISPR editing outcomes with CRISPResso2 (Clement 2019 Nat Biotechnol) across Cas9-nuclease (indels, HDR), CBE and ABE base editors (target conversion + bystander), and prime editor (pegRNA-templated) modes. Covers single-amplicon (CRISPResso), multi-sample batch (CRISPRessoBatch), pooled-amplicon (CRISPRessoPooled), WGS off-target (CRISPRessoWGS), and sample-comparison (CRISPRessoCompare) workflows; quantification-window math that controls what is called edited; substitution-vs-indel diagnostic to distinguish BE from Cas9 contamination; MMEJ deletion pattern interpretation; allele-frequency tables; and failure modes from amplicon misalignment or contamination. Use when quantifying editing from amplicon sequencing, choosing CRISPResso mode by design, distinguishing intended edits from bystanders and indel byproducts, debugging low-alignment runs, or generating publication-grade editing reports.
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Bisulfite sequencing read alignment using Bismark with bowtie2/hisat2. Handles genome preparation and produces BAM files with methylation information. Use when aligning WGBS, RRBS, or other bisulfite-converted sequencing reads to a reference genome.
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Align DNA short reads to reference genomes using bwa-mem2, the faster successor to BWA-MEM. Use when aligning DNA short reads to a reference genome.
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End-to-end ChIP-seq workflow from FASTQ files to annotated peaks. Covers QC, alignment, peak calling with MACS3 (or HOMER), and peak annotation with ChIPseeker. Use when processing ChIP-seq data from alignment through peak annotation.
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Align preprocessed CLIP-seq reads (eCLIP, iCLIP, iCLIP2, PAR-CLIP) to genome with STAR or bowtie2 using crosslink-preserving parameters, choosing between unique-mapper-only and multi-mapper-aware alignment for repeat-binding RBPs, deciding STAR vs HISAT2 memory trade-offs, and applying ENCODE-compatible filters. Use when turning preprocessed CLIP FASTQ into a deduplicated, MAPQ-filtered BAM ready for peak calling or crosslink-site detection.
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Quantify transcript expression using pseudo-alignment with Salmon or kallisto. Use when quantifying transcripts with Salmon or kallisto.
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Create and use BAI/CSI indices for BAM/CRAM files using samtools and pysam. Use when enabling random access to alignment files or fetching specific genomic regions.
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Sort alignment files by coordinate or read name using samtools and pysam. Use when preparing BAM files for indexing, variant calling, or paired-end analysis.
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Trim multiple sequence alignments using ClipKIT, trimAl, BMGE, Divvier, or HMMcleaner with mode selection guidance per downstream goal. Use when removing unreliable columns or contaminating residues before phylogenetic inference, HMM building, or selection analysis.
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Generate alignment statistics using samtools flagstat, stats, depth, coverage, and mosdepth. Use when assessing alignment quality, calculating coverage, or generating QC reports.
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Workflow for gene and transcript quantification from RNA-seq reads using alignment-based or alignment-free tools.
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Think and work like an expert Connectomics Scientist. Use when a task calls for Connectomics Scientist judgment. Reasons from vEM acquisition and petascale alignment through FFN/RoboEM segmentation, FlyWire/neuPrint/MICrONS/H01 graphs, and synapse-level QC while treating split/merge errors, alignment tears, false synapses, and release-version drift as first-class failure modes.
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Design engineering principles for making TUI interfaces feel polished. Use when building Textual widgets, reviewing TUI code, implementing transitions, focus states, alignment, spacing, or any visual detail work. Triggers on TUI polish, design details, "make it feel better", "feels off", layout alignment, color consistency, responsive sizing.
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Create stakeholder alignment artifacts including responsibility matrices, decision frameworks, and communication plans.
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Compares two or more band-tagged frameworks and produces a framework-neutral topic matrix showing coverage and gaps across all inputs, plus an optional reference-centric PLC crosswalk document when a reference framework is supplied.
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Create alignment around your idea before starting the project
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Your team has high autonomy — and standups are vague, weekly meetings drift into status reports, and 1:1s produce nothing but "I'm fine." This playbook fixes all three layers at once. What's inside: • Daily Standup system — 3-field format, good sample, 4 COO principles to keep it honest • Weekly Meeting — strict 25-min agenda, Scrum Master rotation, 0–10 pulse check • 1:1 Framework — 9-dimension quantified pulse, 5-group deep question bank, bilateral feedback structure • Diagnostic layer — "stuck-at" detection: info / authority / collaboration / capability / direction • FAQ for 4 common failure modes (dead standups, status-report meetings, "I'm fine" 1:1s, scaling beyond 10 people) 🇨🇳 你的团队自由度很高——但 standup 越来越糊，例会变成汇报，1:1 问不出真话。这套三层 Motivation 操作手册解决的正是这件事：standup 是信息层，例会是对齐层，1:1 是个人层。三件事做好了，motivation 不是管出来的，是长出来的。 🇯🇵 チームの自律性は高いのに、スタンドアップは曖昧で、週次ミーティングは進捗報告になり、1on1では「大丈夫です」しか返ってこない——このプレイブックは、その3層すべてを同時に解決します。 🇰🇷 팀의 자율성은 높은데 스탠드업은 두루뭉술하고, 주간 회의는 진척 보고가 되어버리고, 1:1에서는 진심을 들을 수 없다면 — 이 플레이북이 세 레이어 모두를 동시에 해결합니다. Triggers: "团队 motivation" | "standup 没人写" | "例会变成汇报" | "1:1 问不出真话" | "高自由度团队管理" | "COO 如何管人" | "team motivation" | "standup best practices" | "1on1 framework" | "weekly meeting template" | "チームモチベーション" | "팀 동기부여" | "AI startup team ops" | "remote team alignment" | "engagement下降"
Implement affective valence as directional derivative of interoceptive energy landscapes for AI alignment. Use when building alignment-aware RL agents, validating GF(3) conservation in reward signals, training Langevin-based policies, or analyzing fold-change detection signals in POMDP environments.
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Define and develop plugin mission statements — purpose, values, anti-patterns, and trade-offs. Use when creating a new plugin, auditing an existing plugin's alignment, or providing a reference for the alignment check loop to evaluate decisions against. Produces mission.json with [draft] status and writes an interview file for the human to refine it.
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