「reading」の検索結果

Quality control for IMC/MIBI data across pixel, channel, image, slide, and batch levels, covering Poisson-count SNR (cell-level Gaussian-mixture and empty-channel comparison), spillover-matrix QC (the three physical sources), drift and the missing EQ-bead analog, acquisition artifacts, and sample-of-origin batch effects. Use when deciding whether to keep or drop a channel, ROI, or slide, distinguishing a dim antibody from a failed one, reading a spillover matrix, or diagnosing batch-driven clustering before analysis.

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Handles BED-format genomic intervals (BED3 through BED12, narrowPeak/broadPeak) and the coordinate-system substrate the whole interval category rests on, with bedtools (CLI) and pybedtools/pyranges/pandas (Python). Covers the 0-based half-open vs 1-based-closed convention boundary and the start-1/end-unchanged conversion, the silent failures (chrom-name mismatch, CRLF, lexicographic-vs-version sort under -sorted), genome/chrom.sizes generation, sorting contracts, BED12 block invariants, validation, makewindows, cross-assembly liftover (liftOver/CrossMap), and BED<->VCF/BAM/FASTA conversion. Use when reading, creating, validating, sorting, lifting between genome builds, or converting interval files, preparing inputs for bedtools/tabix/bigBed, or debugging an off-by-one or empty-overlap result.

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Orders and orients assembled contigs into chromosome-scale scaffolds from long-range linking data, inserting N-gap spacers (adds no sequence). Covers Hi-C/Omni-C scaffolding (YaHS, SALSA2, 3D-DNA/Juicer), Hi-C read-mapping prerequisites (map each end separately, no mate rescue, dedup, enzyme-aware), reading the contact map for misjoins/inversions/false-duplications, manual curation in Juicebox/PretextView (the VGP/DToL standard), reference-guided scaffolding (RagTag) and its karyotype-erasure hazard, genetic-map (ALLMAPS) and Bionano optical-map integration, chimera-breaking before scaffolding, gap-filling, and telomere/contig-vs-scaffold-N50 QC (tidk). Use when turning contigs into chromosomes with Hi-C, integrating a linkage map or optical map, choosing a scaffolder by available linking data, or judging whether a chromosome-scale assembly is trustworthy.

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Designs cytosine (CBE, C-to-T) and adenine (ABE, A-to-G) base-editor guides by positioning the target base at the activity-peak of the editing window (protospacer positions ~5-7, PAM-distal numbering), minimizing bystander edits for product purity, reading dinucleotide context (APOBEC1 TC favored / GC disfavored), and selecting the editor variant (BE4max, ABEmax, ABE8e, YE1/SECURE, TadCBE, CGBE, SpG/SpRY-BE). Covers knockout by premature stop (CRISPR-STOP/iSTOP) and splice-site disruption, the three off-target classes (Cas-dependent, Cas-independent DNA, RNA), outcome prediction (BE-Hive/DeepBE), and the base-vs-prime-vs-HDR decision. Use when installing a transition mutation without a double-strand break, knocking out a gene without indels, or choosing CBE vs ABE. Generic guide scoring, prime editing, and HDR donors are separate skills.

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Corrects fluorophore spillover (conventional compensation) or spectral overlap (spectral unmixing) and applies variance-stabilizing transforms (logicle/biexponential, arcsinh, log) for flow and mass cytometry. Covers spillover-matrix estimation from single-stain controls, AutoSpill, the spillover spreading matrix and why panel design (not compensation) bounds resolution, compensate-then-transform ordering, and arcsinh cofactor choice (5 for CyTOF, ~150 for fluorescence, per-channel via flowVS). Use when correcting spectral overlap, preparing data for gating/clustering, choosing logicle vs arcsinh, deciding a cofactor, or distinguishing compensation from spectral unmixing.

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Read, write, and convert multiple sequence alignment files using Biopython Bio.AlignIO. Supports Clustal, PHYLIP, Stockholm, FASTA, Nexus, and other alignment formats for phylogenetics and conservation analysis. Use when reading, writing, or converting alignment file formats.

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Run build, deploy, inspect, and fix loops for .NET MAUI apps that already have MAUI DevFlow integrated. USE FOR: launching MAUI apps, selecting devices or emulators, waiting for or recovering agent connections, broker/port/adb connectivity issues, visual tree inspection, screenshots, UI interaction, Blazor WebView CDP debugging, reading DevFlow logs, and iterative app debugging. DO NOT USE FOR: first-time DevFlow package setup (use maui-devflow-onboard), or generic desktop automation unrelated to MAUI. INVOKES: maui devflow CLI, dotnet CLI, Android adb/android tools, and Apple simctl tools.

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Startup rules for the app shell, layouts, and global providers. Use when editing app/Providers.tsx, app/DeferredSingletonWrapper.tsx / DeferredSingletonCore.tsx, a layout.tsx, StoreProvider, or a global provider; adding startup fetches or mount-time useEffects high in the tree; or reading auth state (isAdmin, isGuest, fingerprintId) client-side.

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Find and identify the CBasePlayerPawn::CommitSuicide virtual function's vtable slot in the CS2 server binary using IDA Pro MCP. Use this skill when reverse engineering server.dll or libserver.so to locate the self-inflicted-death handler (used by the "kill"/bot_kill console commands and related gameplay code) by RTTI-walking CBasePlayerPawn's primary vtable and reading its known slot index directly, then confirming the body matches CommitSuicide(bool bExplode, bool bForce) semantics. Trigger: CBasePlayerPawn_CommitSuicide

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Gives coding agents temporal awareness by reading the session transcript the host already writes, so the agent stops guessing about time and stops repeating commands that just failed. Activates whenever an agent reasons about recency, elapsed time, retry windows, deploy cooldowns, memory or file staleness, idle detection, or any "when / how long ago / how long did that take" question. Required for autonomous, parallel, or long-running agents.

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When the user requests to add academic papers to a BibTeX bibliography file while maintaining format consistency and sourcing from appropriate repositories. This skill handles 1) Reading existing BibTeX files to understand formatting conventions, 2) Searching for academic papers across multiple sources (OpenReview for conference papers, arXiv for preprints), 3) Extracting proper BibTeX metadata from conference pages or arXiv entries, 4) Determining appropriate citation format (@article vs @inproceedings) based on publication venue, 5) Appending new entries while preserving existing file structure and formatting. Triggers include requests to 'add to ref.bib', 'update bibliography', 'cite papers', or when working with academic reference files.

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X/Twitter API integration for posting tweets, threads, reading timelines, search, and analytics. Covers OAuth auth patterns, rate limits, and platform-native content posting. Use when the user wants to interact with X programmatically.

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Turn a one-line objective into a step-by-step construction plan any coding agent can execute cold. Each step has a self-contained context brief — a fresh agent in a new session can pick up any step without reading prior steps.

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Interact with X/Twitter via the x-cli terminal client using official X API credentials. Use for posting, reading timelines, searching tweets, liking, retweeting, bookmarks, mentions, and user lookups.

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Use this skill any time a .pptx file is involved in any way — as input, output, or both. This includes: creating slide decks, pitch decks, or presentations; reading, parsing, or extracting text from any .pptx file (even if the extracted content will be used elsewhere, like in an email or summary); editing, modifying, or updating existing presentations; combining or splitting slide files; working with templates, layouts, speaker notes, or comments. Trigger whenever the user mentions "deck," "slides," "presentation," or references a .pptx filename, regardless of what they plan to do with the content afterward. If a .pptx file needs to be opened, created, or touched, use this skill.

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Set up and use 1Password CLI (op). Use when installing the CLI, enabling desktop app integration, signing in, and reading/injecting secrets for commands.

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SiYuan Note API for searching, reading, creating, and managing blocks and documents in a self-hosted knowledge base via curl.

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Token-optimized structural code search using tree-sitter AST parsing. Use instead of reading full files when you need to understand code structure, find functions, or explore a codebase efficiently.

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Automatically organizes invoices and receipts for tax preparation by reading messy files, extracting key information, renaming them consistently, and sorting them into logical folders. Turns hours of manual bookkeeping into minutes of automated organization.

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Use this skill any time a .pptx file is involved in any way — as input, output, or both. This includes: creating slide decks, pitch decks, or presentations; reading, parsing, or extracting text from any .pptx file (even if the extracted content will be used elsewhere, like in an email or summary); editing, modifying, or updating existing presentations; combining or splitting slide files; working with templates, layouts, speaker notes, or comments. Trigger whenever the user mentions "deck," "slides," "presentation," or references a .pptx filename, regardless of what they plan to do with the content afterward. If a .pptx file needs to be opened, created, or touched, use this skill.

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Comprehensive spreadsheet creation, editing, and analysis with support for formulas, formatting, data analysis, and visualization. When Claude needs to work with spreadsheets (.xlsx, .xlsm, .csv, .tsv, etc) for: (1) Creating new spreadsheets with formulas and formatting, (2) Reading or analyzing data, (3) Modify existing spreadsheets while preserving formulas, (4) Data analysis and visualization in spreadsheets, or (5) Recalculating formulas

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Use this skill whenever the user wants to do anything with PDF files. This includes reading or extracting text/tables from PDFs, combining or merging multiple PDFs into one, splitting PDFs apart, rotating pages, adding watermarks, creating new PDFs, filling PDF forms, encrypting/decrypting PDFs, extracting images, and OCR on scanned PDFs to make them searchable. If the user mentions a .pdf file or asks to produce one, use this skill.

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Calculate comprehensive numerology reports using Pythagorean or Chaldean systems. Supports English, Tamil, Telugu, Kannada, and Hindi. Use when a user wants numerology readings, life path numbers, destiny numbers, name analysis, birth date numerology, or lucky number calculations.

日本語の概要は準備中です。原文の説明を表示しています。

中国传统面相学 v2.0.0 - 面相心理学专家：融合传统面相、AI图像识别与心理学分析，既看面相更看改善

日本語の概要は準備中です。原文の説明を表示しています。