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Optimize test suites using mutation testing to maximize mutation kill rate with minimal tests. Use when you need to reduce test suite size while maintaining quality, identify redundant tests, improve mutation coverage, or validate test effectiveness. The skill analyzes test coverage, execution intervals, and redundancy using mutation operators, selects or generates a minimal subset of tests that maximizes mutation kill rate, and outputs an optimized test suite with detailed reports showing killed and surviving mutants. Supports multiple programming languages and mutation testing frameworks.

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| Type-safe ORM for Cloudflare D1 databases using Drizzle. Use when: building D1 database schemas, writing type-safe SQL queries, managing migrations with Drizzle Kit, defining table relations, implementing prepared statements, using D1 batch API, or encountering D1_ERROR, transaction errors, foreign key constraint failures, or schema inference issues.

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Detects somatic mutations in circulating tumor DNA using variant callers optimized for low allele fractions with UMI-based error suppression. Reliably detects mutations at VAF above 0.5 percent using consensus-based approaches. Use when identifying tumor mutations from plasma DNA or tracking specific variants.

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Automated cell type annotation using reference-based methods including CellTypist, scPred, SingleR, and Azimuth for consistent, reproducible cell labeling. Use when automatically annotating cell types using reference datasets.

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Transfer gene annotations between genome assemblies using Liftoff for same-species annotation liftover and MiniProt for cross-species protein-to-genome alignment. Enables rapid annotation of new assemblies using existing reference annotations. Use when annotating a new assembly of a species with an existing reference annotation or mapping annotations across related species.

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Calculates species richness, diversity, and turnover using the Hill number framework with iNEXT coverage-based rarefaction/extrapolation, asymptotic diversity estimation, and beta diversity partitioning (betapart turnover vs nestedness). Compares assemblages using coverage-standardized rather than size-standardized rarefaction. Use when quantifying biodiversity from species abundance or incidence data, comparing diversity across sites, or constructing rarefaction curves. Not for clinical 16S microbiome alpha/beta diversity (see microbiome/diversity-analysis).

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Maps query single-cell data to reference atlases using scArches transfer learning with scVI and scANVI models. Transfers cell type labels without retraining on combined data. Use when annotating new single-cell datasets using pre-trained reference models.

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End-to-end binder design using BindCraft hallucination. Use this skill when: (1) Designing protein binders with built-in AF2 validation, (2) Running production-quality binder campaigns, (3) Using different design protocols (fast, default, slow), (4) Need joint backbone and sequence optimization, (5) Want high experimental success rate. For backbone-only generation, use rfdiffusion. For QC thresholds, use protein-qc. For tool selection guidance, use binder-design.

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All-atom protein design using BoltzGen diffusion model. Use this skill when: (1) Need side-chain aware design from the start, (2) Designing around small molecules or ligands, (3) Want all-atom diffusion (not just backbone), (4) Require precise binding geometries, (5) Using YAML-based configuration. For backbone-only generation, use rfdiffusion. For sequence-only design, use proteinmpnn. For structure validation, use boltz.

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Structure prediction using Boltz-1/Boltz-2, an open biomolecular structure predictor. Use this skill when: (1) Predicting protein complex structures, (2) Validating designed binders, (3) Need open-source alternative to AF2, (4) Predicting protein-ligand complexes, (5) Using local GPU resources. For QC thresholds, use protein-qc. For AlphaFold2 prediction, use alphafold. For Chai prediction, use chai.

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Structure prediction using Chai-1, a foundation model for molecular structure. Use this skill when: (1) Predicting protein-protein complex structures, (2) Validating designed binders, (3) Predicting protein-ligand complexes, (4) Using the Chai API for high-throughput prediction, (5) Need an alternative to AlphaFold2. For QC thresholds, use protein-qc. For AlphaFold2 prediction, use alphafold. For ESM-based analysis, use esm.

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Embedded hardware debugging workflow for probe-rs targets using embedded-debugger-mcp. Use when Codex or Claude Code needs to inspect debug probes, validate embedded debugger setup, start the MCP server, guide a user through ARM Cortex-M/RISC-V flashing/debugging/RTT workflows, or operate without installing an MCP client by using the CLI plus prompts.

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Build Spring Boot 4.x applications following the best practices. Use this skill: * When developing Spring Boot applications using Spring MVC, Spring Data JPA, Spring Modulith, Spring Security * To create recommended Spring Boot package structure * To implement REST APIs, entities/repositories, service layer, modular monoliths * To use Thymeleaf view templates for building web applications * To write tests for REST APIs and Web applications * To write ArchUnit tests for testing architecture * To configure the recommended plugins and configurations to improve code quality, and testing while using Maven. * To use Spring Boot's Docker Compose support for local development * To create Taskfile for easier execution of common tasks while working with a Spring Boot application

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Run autonomous AI-driven penetration tests on web applications using tools like Shannon, PentAGI, and similar frameworks. Use when tasks involve setting up automated penetration testing pipelines, combining AI agents with security tools (nmap, subfinder, nuclei, sqlmap), building autonomous exploit chains, generating pentest reports with proof-of-concept exploits, or integrating AI pentesting into CI/CD pipelines. Covers the full pentest lifecycle from reconnaissance to reporting using AI orchestration.

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Azure AI Content Safety SDK for Java workflow skill. Use this skill when the user needs Build content moderation applications using the Azure AI Content Safety SDK for Java and the operator should preserve the upstream workflow, copied support files, and provenance before merging or handing off.

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Azure Document Intelligence (Form Recognizer) SDK for Java workflow skill. Use this skill when the user needs Build document analysis applications using the Azure AI Document Intelligence SDK for Java and the operator should preserve the upstream workflow, copied support files, and provenance before merging or handing off.

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Azure Document Intelligence (Form Recognizer) SDK for Java workflow skill. Use this skill when the user needs Build document analysis applications using the Azure AI Document Intelligence SDK for Java and the operator should preserve the upstream workflow, copied support files, and provenance before merging or handing off.

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Azure AI Content Safety SDK for Java workflow skill. Use this skill when the user needs Build content moderation applications using the Azure AI Content Safety SDK for Java and the operator should preserve the upstream workflow, copied support files, and provenance before merging or handing off.

日本語の概要は準備中です。原文の説明を表示しています。

Avalonia Layout with Zafiro.Avalonia workflow skill. Use this skill when the user needs Guidelines for modern Avalonia UI layout using Zafiro.Avalonia, emphasizing shared styles, generic components, and avoiding XAML redundancy and the operator should preserve the upstream workflow, copied support files, and provenance before merging or handing off.

日本語の概要は準備中です。原文の説明を表示しています。

Avalonia Layout with Zafiro.Avalonia workflow skill. Use this skill when the user needs Guidelines for modern Avalonia UI layout using Zafiro.Avalonia, emphasizing shared styles, generic components, and avoiding XAML redundancy and the operator should preserve the upstream workflow, copied support files, and provenance before merging or handing off.

日本語の概要は準備中です。原文の説明を表示しています。

Lead Generation workflow skill. Use this skill when the user needs Scrape leads from multiple platforms using Apify Actors and the operator should preserve the upstream workflow, copied support files, and provenance before merging or handing off.

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Lead Generation workflow skill. Use this skill when the user needs Scrape leads from multiple platforms using Apify Actors and the operator should preserve the upstream workflow, copied support files, and provenance before merging or handing off.

日本語の概要は準備中です。原文の説明を表示しています。

Agent Framework Azure Hosted Agents workflow skill. Use this skill when the user needs Build persistent agents on Azure AI Foundry using the Microsoft Agent Framework Python SDK and the operator should preserve the upstream workflow, copied support files, and provenance before merging or handing off.

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Agent Framework Azure Hosted Agents workflow skill. Use this skill when the user needs Build persistent agents on Azure AI Foundry using the Microsoft Agent Framework Python SDK and the operator should preserve the upstream workflow, copied support files, and provenance before merging or handing off.

日本語の概要は準備中です。原文の説明を表示しています。