56 件 ・ 関連度順
概要と使いどころ
Block writes through filesystem junctions and symlinks — file write through .mxm-system or .claude junction, git commit through a junction, delete through a junction. Enforces the junction read-only rule so the real repo stays the source of truth.
日本語の概要は準備中です。原文の説明を表示しています。
Assesses RNA-seq data quality specifically for alternative splicing analysis. QC layers include experimental design audit (library prep, read length, depth, replicates), STAR 2-pass cohort-style alignment, junction saturation curves and discovery plateau detection, novel-vs-known junction ratio diagnostics, junction-overhang distribution, splice-site strength scoring (MaxEntScan intrinsic + SpliceAI context-aware), strandedness verification, GENCODE basic vs comprehensive choice, and rRNA contamination screening. Splicing analysis is more demanding than DGE on read length, depth, library prep, alignment strategy, and annotation choice — failures silently bias PSI estimates and inflate novel-junction false positives. Use when evaluating data suitability for splicing analysis, troubleshooting low event detection, or designing sequencing experiments where AS is a primary endpoint.
日本語の概要は準備中です。原文の説明を表示しています。
Assesses RNA-seq data quality specifically for alternative splicing analysis. QC layers include experimental design audit (library prep, read length, depth, replicates), STAR 2-pass cohort-style alignment, junction saturation curves and discovery plateau detection, novel-vs-known junction ratio diagnostics, junction-overhang distribution, splice-site strength scoring (MaxEntScan intrinsic + SpliceAI context-aware), strandedness verification, GENCODE basic vs comprehensive choice, and rRNA contamination screening. Splicing analysis is more demanding than DGE on read length, depth, library prep, alignment strategy, and annotation choice — failures silently bias PSI estimates and inflate novel-junction false positives. Use when evaluating data suitability for splicing analysis, troubleshooting low event detection, or designing sequencing experiments where AS is a primary endpoint.
日本語の概要は準備中です。原文の説明を表示しています。
Explores a CARLA map's road network through the Map/Waypoint API — a natural-language rundown of the whole map ("give me the topology"), drawing the topology to confirm the modelled map matches the visible one, enumerating junctions to resolve phrases like "the 4-way junction in the middle", nearest-waypoint lane facts, and next/previous lane navigation. Use when the user asks about the map's layout, lanes, junctions, topology, or to locate/navigate specific map elements.
日本語の概要は準備中です。原文の説明を表示しています。
Splice-aware RNA-seq aligner producing sorted BAM and splice junction tables. Builds genome index, runs two-pass alignment for better junctions. Outputs sorted BAM, junctions (SJ.out.tab), stats (Log.final.out), optional gene counts. Use Salmon for fast pseudoalignment; STAR when a BAM is needed for variant calling, IGV, or ENCODE pipelines.
日本語の概要は準備中です。原文の説明を表示しています。
Explores a CARLA map's road network through the Map/Waypoint API — a natural-language rundown of the whole map ("give me the topology"), drawing the topology to confirm the modelled map matches the visible one, enumerating junctions to resolve phrases like "the 4-way junction in the middle", nearest-waypoint lane facts, and next/previous lane navigation. Use when the user asks about the map's layout, lanes, junctions, topology, or to locate/navigate specific map elements.
日本語の概要は準備中です。原文の説明を表示しています。
Discovers, classifies, filters, and quantifies full-length transcript isoforms from PacBio Iso-Seq/Kinnex (HiFi) and Oxford Nanopore (cDNA/direct-RNA) long reads, using the isoseq+pigeon pipeline, SQANTI3, and ONT tools (IsoQuant, FLAIR, Bambu, StringTie2). Covers why a novel isoform is an artifact until proven otherwise (RT template-switching, intra-priming, and 5' degradation manufacture junctions and truncations), the SQANTI3 structural categories and their trust order, the Kinnex skera-split step, orthogonal CAGE/poly-A/short-read-junction validation, and why long-read isoform quantification needs EM. Use when building a full-length isoform catalog, classifying/filtering long-read transcripts, running Iso-Seq or ONT cDNA/dRNA analysis, or judging novel-isoform reliability.
日本語の概要は準備中です。原文の説明を表示しています。
Detects differential alternative splicing between conditions using rMATS-turbo (binomial LRT on junction counts), leafcutter (Dirichlet-multinomial GLM on intron clusters), MAJIQ V3 deltapsi/HET (Bayesian posterior on LSVs), SUPPA2 (empirical-null on TPM-derived PSI), or Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage). Reports FDR-corrected significance and delta PSI effect sizes. Tools differ in statistical model, annotation dependence, calibration regime, and replicate-count requirements. Use when comparing splicing patterns between treatment groups, tissues, or disease states.
日本語の概要は準備中です。原文の説明を表示しています。
Analyzes alternative splicing at single-cell resolution. The first decision is library chemistry — 10X 3' is fundamentally limited (RT primes from poly-A, R2 falls in 3' UTR, <0.1 junction read per cell per AS event). Plate-based full-length methods (Smart-seq3, FLASH-seq, VASA-seq, STORM-seq) and single-cell long-read (MAS-Iso-seq, scISOr-Seq2) are the chemistries that give per-cell isoform structure. Tools include MARVEL (R, Smart-seq integrated), BRIE2 (Bayesian PSI with regulatory features and ELBO_gain test), scQuint (junction-cluster, plate-based; not for 10X), SpliZ (annotation-free Z-score), Psix (graph-smoothness regulated AS), and Sierra (alternative polyadenylation, often confused with AS). Use when analyzing isoform usage in scRNA-seq, identifying cell-type-specific splicing, or determining whether scRNA-seq chemistry supports splicing analysis at all.
日本語の概要は準備中です。原文の説明を表示しています。
在Python 2.7环境下解析Windows目录链接（Junction）或符号链接的真实物理路径。提供基于PyWin32 API和PyQt4/5 QProcess调用cmd命令的两种解决方案，解决os.path.realpath失效及输出解析问题。
日本語の概要は準備中です。原文の説明を表示しています。
Detects differential alternative splicing between conditions using rMATS-turbo (binomial LRT on junction counts), leafcutter (Dirichlet-multinomial GLM on intron clusters), MAJIQ V3 deltapsi/HET (Bayesian posterior on LSVs), SUPPA2 (empirical-null on TPM-derived PSI), or Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage). Reports FDR-corrected significance and delta PSI effect sizes. Tools differ in statistical model, annotation dependence, calibration regime, and replicate-count requirements. Use when comparing splicing patterns between treatment groups, tissues, or disease states.
日本語の概要は準備中です。原文の説明を表示しています。
Physical principles underlying diodes, bipolar junction transistors, and MOSFETs — carrier concentrations, drift and diffusion, the pn junction, forward and reverse bias, the Shockley diode equation, BJT operating regions, MOSFET threshold and saturation, small-signal models, and temperature effects. Use when reasoning about device-level behavior, designing bias networks, selecting transistor operating points, interpreting datasheets, or diagnosing nonlinear device failures.
日本語の概要は準備中です。原文の説明を表示しています。
Adjunctions as universal arrows. Left ⊣ Right.
日本語の概要は準備中です。原文の説明を表示しています。
Detects differential alternative splicing between conditions using rMATS-turbo (binomial LRT on junction counts), leafcutter (Dirichlet-multinomial GLM on intron clusters), MAJIQ V3 deltapsi/HET (Bayesian posterior on LSVs), SUPPA2 (empirical-null on TPM-derived PSI), or Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage). Reports FDR-corrected significance and delta PSI effect sizes. Tools differ in statistical model, annotation dependence, calibration regime, and replicate-count requirements. Use when comparing splicing patterns between treatment groups, tissues, or disease states.
日本語の概要は準備中です。原文の説明を表示しています。
Analyzes alternative splicing at single-cell resolution. The first decision is library chemistry — 10X 3' is fundamentally limited (RT primes from poly-A, R2 falls in 3' UTR, <0.1 junction read per cell per AS event). Plate-based full-length methods (Smart-seq3, FLASH-seq, VASA-seq, STORM-seq) and single-cell long-read (MAS-Iso-seq, scISOr-Seq2) are the chemistries that give per-cell isoform structure. Tools include MARVEL (R, Smart-seq integrated), BRIE2 (Bayesian PSI with regulatory features and ELBO_gain test), scQuint (junction-cluster, plate-based; not for 10X), SpliZ (annotation-free Z-score), Psix (graph-smoothness regulated AS), and Sierra (alternative polyadenylation, often confused with AS). Use when analyzing isoform usage in scRNA-seq, identifying cell-type-specific splicing, or determining whether scRNA-seq chemistry supports splicing analysis at all.
日本語の概要は準備中です。原文の説明を表示しています。
Detects differential alternative splicing between conditions using rMATS-turbo (binomial LRT on junction counts), leafcutter (Dirichlet-multinomial GLM on intron clusters), MAJIQ V3 deltapsi/HET (Bayesian posterior on LSVs), SUPPA2 (empirical-null on TPM-derived PSI), or Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage). Reports FDR-corrected significance and delta PSI effect sizes. Tools differ in statistical model, annotation dependence, calibration regime, and replicate-count requirements. Use when comparing splicing patterns between treatment groups, tissues, or disease states.
日本語の概要は準備中です。原文の説明を表示しています。
在Python 2.7环境下解析Windows目录链接（Junction）或符号链接的真实物理路径。提供基于PyWin32 API和PyQt4/5 QProcess调用cmd命令的两种解决方案，解决os.path.realpath失效及输出解析问题。
日本語の概要は準備中です。原文の説明を表示しています。
Designs and audits PCR and RT-qPCR primers with Primer3, explicit thermodynamic conditions, reference-based off-target amplification searches, and traceable sequence coordinates. Use for designing primer pairs, checking existing primers, exon-junction or isoform-specific assays, variant masking, cloning tails, multiplex compatibility, and interpreting Primer-BLAST results. Includes bounded local in-silico PCR and BLAST screening; distinguishes computational candidates from experimentally validated assays.
日本語の概要は準備中です。原文の説明を表示しています。
Runs a route through ScenarioRunner's RouteScenario mode (--route / --route-id) with an autonomous agent driving the ego — the same mechanism the CARLA Leaderboard uses, and the only way to reach the ~34 route-only scenario classes (Accident, ParkedObstacle, HazardAtSideLane, InvadingTurn, HardBreakRoute, YieldToEmergencyVehicle, junction and actor-flow scenarios). Inspects route files, lists route ids and their scenarios, and runs one or all of them. Use when the user asks to "run a route", "drive a route with my agent", "run leaderboard scenarios without the leaderboard", or names a route-only scenario type.
日本語の概要は準備中です。原文の説明を表示しています。
Drives a vehicle to a destination autonomously using CARLA's navigation agents (BasicAgent/BehaviorAgent/ConstantVelocityAgent), or plans a route between two points with GlobalRoutePlanner. The agent handles routing, obstacle avoidance and traffic-light obedience. Use when the user asks to "drive the ego to (x,y,z)", "navigate to the junction", "go to that location", "plan a route from A to B", or "make the car drive itself somewhere specific". Manual control is control-vehicle; autopilot roaming is control-traffic.
日本語の概要は準備中です。原文の説明を表示しています。
Controls the traffic-light actors on a running CARLA server — set a light green/red/yellow/off, freeze all lights (optionally all-green), set phase timing (green/yellow/red durations), and reset to the normal cycle. Target all lights, one by id, all in a junction, or the nearest. Use when the user asks to "make the light red", "freeze all lights green", "change the light timing", "turn the traffic lights off", or "reset the lights". This is the light actors, not the Traffic Manager (that's control-traffic).
日本語の概要は準備中です。原文の説明を表示しています。
Runs a single ScenarioRunner Python scenario (or a whole group) against a running CARLA server — FollowLeadingVehicle, ControlLoss, DynamicObjectCrossing, SignalizedJunctionLeftTurn and the rest of srunner/examples — in sync or async mode, with result output, recording and manual/agent control of the ego. Lists what is runnable on the checked-out branch and on the loaded map. Use when the user asks to "run a scenario", "test FollowLeadingVehicle", "list scenarios", "run a CARLA traffic scenario", or reports a scenario that is "not supported".
日本語の概要は準備中です。原文の説明を表示しています。
Score, annotate, and analyze the functional impact of genetic variants using AlphaGenome Variant Impact (AVI) scores. Query variants in chr:pos:ref>alt format, annotate VCF/tabular callsets, perform saturation mutagenesis window scans (1-based closed chr:start-end), and extract GENCODE v46 GTF gene/exon/junction coordinates all via the AlphaGenome Atlas API.
日本語の概要は準備中です。原文の説明を表示しています。
Verify skillshare on a real Windows guest running in local UTM: build a pinned commit in the devcontainer, push it into the VM, and either run the Windows E2E runbook with full and basic-user tokens or hand the maintainer a one-line hands-on setup. Use this whenever a change touches Windows links, junctions, file symlinks, Developer Mode, copy fallback, Windows paths, or the dashboard on Windows, or when the user asks to test or accept something on Windows / UTM.
日本語の概要は準備中です。原文の説明を表示しています。