生成图文并茂的分析报告/实验报告/质检报告(固定模版)。当用户要求"生成报告/总结报告/实验报告/质检报告/QC报告",或分析流程收尾需要交付报告时使用。报告必须基于真实分析输出,包含固定章节结构、关键指标表格和真实结果图。
日本語の概要は準備中です。原文の説明を表示しています。
Plot per-group distributions of continuous data using boxplots, violins, beeswarms, quasirandom jitter, and raincloud plots with sample-size honesty (Weissgerber 2015), KDE-bandwidth awareness, and N-aware encoding choices. Use when comparing distributions across a small number of groups — expression per cluster, biomarker per arm, scores per condition — and the bar-of-mean default is misleading.
インストールする前に、エージェントに与えられる指示の中身を確認できます。
Reference examples tested with: ggplot2 3.5+, ggbeeswarm 0.7+, ggdist 3.3+, gghalves 0.1.4+, seaborn 0.13+, matplotlib 3.8+, ptitprince 0.3+ (Python raincloud).
Before using code patterns, verify installed versions match. If versions differ:
pip show <package> then help(module.function) to check signaturespackageVersion('<pkg>') then ?function_name to verify parametersIf code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.
"Plot the distribution per group" -> Render boxplot, violin, beeswarm, or raincloud calibrated to N per group, the underlying distribution shape, and the audience's ability to read each encoding. The default geom_bar(stat='summary') is the canonical misleading choice — Weissgerber 2015 PLOS Biol documented that 703 top physiology papers use bar-of-mean despite multiple distinct distributions producing identical bars.
ggplot2::geom_boxplot, ggplot2::geom_violin, ggbeeswarm::geom_quasirandom, ggdist::stat_halfeye, gghalves::geom_half_violinseaborn.boxplot/violinplot/swarmplot/stripplot, ptitprince.RainCloudWeissgerber, Milic, Winham & Garovic 2015 PLOS Biol 13:e1002128 surveyed 703 papers in top physiology journals and found that bar-and-line graphs of means dominate, despite many distinct distributions producing identical bar plots. Bimodal data, skewed data, and data with outliers all collapse to the same bar height and error bar. The bar plot is a hypothesis test result rendered as visualization; the visualization should show the data.
The modern alternative is to show every point for n < 30, layer summary on top, and reserve summary-only plots for large N where points would overplot.
| N per group | Recommended | Avoid |
|---|---|---|
| 3-10 | Dot plot or jittered raw points + median bar | Bar of mean |
| 10-30 | Beeswarm OR quasirandom + box overlay | Bare boxplot (hides bimodality) |
| 30-200 | Raincloud (Allen 2019) OR box + jitter | Bare violin (default KDE bandwidth oversmooths) |
| 200-1000 | Letter-value plot (Hofmann 2017) OR violin with explicit bandwidth | Box alone (collapses tails) |
| >1000 | Density (KDE) or histogram + summary stats | Individual points (overplot) |
Always annotate N somewhere on the plot (caption, x-axis tick label, or stratum count).
ggplot(df, aes(group, value, fill = group)) +
geom_boxplot(outlier.shape = NA, alpha = 0.7, width = 0.5) +
geom_jitter(width = 0.2, alpha = 0.5, size = 1) +
scale_fill_manual(values = c('#0072B2', '#D55E00')) +
labs(x = NULL, y = 'Expression') +
theme_classic()
Box shows: median, IQR, 1.5×IQR whiskers, outliers. Hides: bimodality, sample size, density.
Notched boxplot (notch = TRUE): notches show 95% CI for median (±1.58·IQR/√n); non-overlapping notches roughly indicate distinct medians. Use with N ≥ 15.
ggplot(df, aes(group, value, fill = group)) +
geom_violin(alpha = 0.7, trim = FALSE,
bw = 'SJ') + # Sheather-Jones bandwidth
geom_boxplot(width = 0.1, fill = 'white', outlier.shape = NA) +
scale_fill_manual(values = c('#0072B2', '#D55E00'))
KDE bandwidth pitfall: ggplot's default is Silverman's rule of thumb, which oversmooths bimodal data into a single mode. Use bw = 'SJ' (Sheather-Jones plug-in) for honest representation of multimodality.
trim = TRUE (default) cuts the violin at the data range — visually misleading because the violin's tails imply density extending beyond the data. trim = FALSE lets the KDE extend.
library(ggbeeswarm)
ggplot(df, aes(group, value, color = group)) +
geom_quasirandom(method = 'quasirandom', width = 0.3, alpha = 0.7) +
scale_color_manual(values = c('#0072B2', '#D55E00')) +
stat_summary(fun = median, geom = 'crossbar', width = 0.5, color = 'black')
Quasirandom (van der Corput sequence; Bostock implementation) gives reproducible jitter that fills space without random scatter. Beeswarm is similar but with collision avoidance. Both are deterministic — reruns produce identical layouts.
Goal: Show distribution (half-violin), summary (boxplot), and raw observations (jittered points) in a single per-group panel without occlusion.
Approach: Place a half-violin on one side, a thin boxplot in the middle, and jittered points on the other side via gghalves::geom_half_violin + geom_boxplot + geom_half_point with position_nudge offsets; flip to horizontal so the visual reads as a literal raincloud.
library(gghalves)
ggplot(df, aes(group, value, fill = group, color = group)) +
geom_half_violin(side = 'r', alpha = 0.7, position = position_nudge(x = 0.15)) +
geom_boxplot(width = 0.15, outlier.shape = NA, alpha = 0.7,
position = position_nudge(x = -0.05)) +
geom_half_point(side = 'l', alpha = 0.5, size = 1.5, range_scale = 0.4,
position = position_nudge(x = -0.2)) +
scale_fill_manual(values = c('#0072B2', '#D55E00')) +
scale_color_manual(values = c('#0072B2', '#D55E00')) +
coord_flip() # horizontal "raincloud"
import ptitprince as pt
import seaborn as sns
pt.RainCloud(x='group', y='value', data=df,
palette=['#0072B2', '#D55E00'],
bw='scott', cut=0, # bandwidth + trim
width_viol=0.6, orient='h')
Raincloud = half-violin (distribution) + boxplot (summary) + jittered raw points. Allen 2019 Wellcome Open Res 4:63 — modern publication default for N 30-200.
library(lvplot)
ggplot(df, aes(group, value, fill = group)) +
geom_lv(k = 5, alpha = 0.7) +
scale_fill_manual(values = c('#0072B2', '#D55E00'))
Extends Tukey's boxplot via additional letter-value quantiles (Hofmann, Wickham, Kafadar 2017 J Comput Graph Stat 26:469). For large N, the standard boxplot collapses tail structure; letter-value preserves it.
sns.boxenplot(x='group', y='value', data=df,
palette=['#0072B2', '#D55E00']) # seaborn calls it boxenplot
library(introdataviz) # split-violin geom
ggplot(df, aes(group, value, fill = condition)) +
geom_split_violin(alpha = 0.7) +
geom_boxplot(width = 0.15, position = position_dodge(0.5), outlier.shape = NA)
For 2-condition comparison within each group, split-violin shows both densities back-to-back. More compact than dodged violins.
Trigger: geom_bar(stat = 'summary') + geom_errorbar(stat = 'summary', fun.data = mean_se).
Mechanism: Mean ± SEM collapses all distributional information; reader cannot assess bimodality, skew, or N.
Symptom: Reviewer asks to "show the data"; the figure must be redone.
Fix: Replace with raincloud, beeswarm, or boxplot+jitter. Show points for N < 30.
Trigger: geom_violin() without specifying bw.
Mechanism: Silverman's rule of thumb assumes unimodal Gaussian; oversmooths bimodal data into a single peak.
Symptom: Single-cell expression bimodality (off / on) renders as a unimodal violin; biologically false.
Fix: bw = 'SJ' (Sheather-Jones plug-in) for honest bimodality. Note: nrd0 IS Silverman; nrd (Scott) oversmooths less than Silverman but Sheather-Jones is preferred.
Trigger: notch = TRUE with N < 15 per group.
Mechanism: Notch can extend beyond Q1/Q3, producing visually-misleading "inside-out" notches.
Symptom: ggplot warning ("notch went outside hinges"); notches look weird.
Fix: Use notches only with N ≥ 15. For smaller N, show raw points instead.
Trigger: geom_boxplot() + geom_jitter() with default outlier.shape = 19.
Mechanism: Outliers render twice — once from boxplot (large dots), once from jitter (smaller dots) — visually duplicated.
Symptom: Some points appear bigger than others without reason.
Fix: geom_boxplot(outlier.shape = NA) when overlaying raw points.
Trigger: geom_violin() default trim = TRUE.
Mechanism: Default trims violin at the data range; the visual still shows narrowing "tails" implying density extends slightly beyond the data.
Symptom: Reader infers density beyond observed range.
Fix: trim = FALSE to let KDE extend, OR explicitly cap with coord_cartesian. Document the choice.
Trigger: Boxplot with no N per group reported.
Mechanism: Reader cannot assess statistical power; tiny N looks identical to large N at this encoding.
Symptom: Reviewer requests "show N per group."
Fix: Add N to x-axis tick label (Control (n=12)) or use stat_summary(geom='text', fun.data = function(x) data.frame(label = paste('n=', length(x)))).
Trigger: Raincloud applied with N = 5 per group.
Mechanism: KDE with N=5 is meaningless; violin shape is artifact of bandwidth.
Symptom: Smooth violin from 5 points; misleads about underlying distribution.
Fix: For N < 30, drop the violin half; use box + raw points only.
| Pattern | Cause | Action |
|---|---|---|
| Bar of mean shows clear separation; raincloud shows overlapping distributions | Bars hide overlap | Use raincloud; bars exaggerate effect |
| Violin shows unimodal; histogram shows bimodal | Default Silverman bandwidth oversmooths | Re-render with bw = 'SJ' |
| Boxplot medians look distinct; t-test n.s. | Boxplot of small N is unreliable | Show raw points; rerun with appropriate non-parametric test |
| Notched boxplot notches non-overlap; rank test n.s. | Notch is an approximation, not a hypothesis test | Notches are heuristic only; use formal test |
Operational rule: for N < 30, show every point. For N 30-200, raincloud. For N > 200, letter-value or violin with explicit bandwidth. Always annotate N.
| Threshold | Value | Source |
|---|---|---|
| N for valid notched boxplot | ≥15 | Common practice |
| N to show raw points | <30 | Weissgerber 2015 |
| N where violin > box | >30 (with explicit bandwidth) | Visualization guidance |
| Whisker length (Tukey) | 1.5 × IQR | Tukey 1977 |
| Notch length (McGill 1978) | ±1.58 × IQR / sqrt(N) | McGill 1978 |
| KDE bandwidth (Sheather-Jones) | plug-in selector | Sheather-Jones 1991 |
| Error / symptom | Cause | Solution |
|---|---|---|
| Bimodal data shown as unimodal violin | Default Silverman bandwidth | bw = 'SJ' |
| Duplicate large points on box + jitter | outlier.shape not suppressed | geom_boxplot(outlier.shape = NA) |
| Notches "inside-out" | N too small | Show raw points; remove notch |
| Raincloud looks smooth at N=5 | KDE meaningless at small N | Drop violin half; box + points only |
| No N visible | Default boxplot | Add n=... to x label or stat_summary text |
| Violin tails extend beyond data | trim = TRUE default + KDE bandwidth | trim = FALSE and cap with coord_cartesian |
| Bar of mean criticized in review | Weissgerber 2015 default failure | Replace with raincloud or box+jitter |
まだレビューはありません。使ってみた感想をお寄せください。
概要と使いどころ
生成图文并茂的分析报告/实验报告/质检报告(固定模版)。当用户要求"生成报告/总结报告/实验报告/质检报告/QC报告",或分析流程收尾需要交付报告时使用。报告必须基于真实分析输出,包含固定章节结构、关键指标表格和真实结果图。
日本語の概要は準備中です。原文の説明を表示しています。
Predicts ADMET properties using ADMETlab 3.0 (119 endpoints with uncertainty), ADMET-AI, DeepChem MolNet, and chemprop D-MPNN with explicit handling of OECD QSAR principles, applicability domain assessment, calibration, hERG/CYP/AMES gold-standard endpoints, and PAINS / Lipinski / Ro5 / Veber / BBB druglikeness filters. Use when filtering compounds for drug-likeness, prioritizing leads by predicted safety, or building an in-house ADMET QSAR model.
日本語の概要は準備中です。原文の説明を表示しています。
Trim PCR primers from aligned reads in amplicon-panel BAMs using samtools ampliconclip. Use when processing SARS-CoV-2 ARTIC, hereditary cancer panels, ctDNA hot-spot panels, or any amplicon assay where primer-derived bases would falsely confirm reference at primer footprints.
日本語の概要は準備中です。原文の説明を表示しています。
Filter alignments by flags, mapping quality, and regions using samtools view and pysam. Use when extracting specific reads, removing low-quality alignments, or subsetting to target regions.
日本語の概要は準備中です。原文の説明を表示しています。
Create and use BAI/CSI indices for BAM/CRAM files using samtools and pysam. Use when enabling random access to alignment files or fetching specific genomic regions.
日本語の概要は準備中です。原文の説明を表示しています。
Read, write, and convert multiple sequence alignment files using Biopython Bio.AlignIO. Supports Clustal, PHYLIP, Stockholm, FASTA, Nexus, and other alignment formats for phylogenetics and conservation analysis. Use when reading, writing, or converting alignment file formats.
日本語の概要は準備中です。原文の説明を表示しています。