19 件 ・ 関連度順
概要と使いどころ
Personal GTD (Getting Things Done) coach that helps users achieve their annual goals. Use when users mention goals, plans, daily tasks, productivity, time management, task tracking, reviews, or need help breaking down objectives into actionable steps. Automatically remembers user's goals and progress across conversations. Supports multiple languages. 新手引导：输入 `/gtd 新手`、「这个怎么用」「第一次用」「能干嘛」时，走 SKILL.md 的〈新手上路〉，不要直接开始干活。
日本語の概要は準備中です。原文の説明を表示しています。
Compute genome-to-genome distances (ANI, AAI, dDDH, k-mer Mash) and assign taxonomic classifications using skani (Shaw 2023), FastANI (Jain 2018), pyani / pyANI ANIb / ANIm, OrthoANI (Lee 2016), AAI (amino-acid identity), dDDH via TYGS / GGDC, GTDB-Tk (Chaumeil 2020 standard prokaryote taxonomy), and Mash MinHash (Ondov 2016). Use when delineating prokaryote species (95% ANI threshold; Jain 2018 Nat Commun 9:5114), assigning genomes to GTDB taxonomy with ANI radius, computing genome similarity matrices for clustering, classifying archaea, evaluating MAG (metagenome-assembled genome) species assignment, applying skani for fast metagenomic ANI screening, or reconciling 16S rRNA-based taxonomy with whole-genome ANI.
日本語の概要は準備中です。原文の説明を表示しています。
Generate an energy-optimized, time-blocked daily plan based on circadian rhythm research and GTD principles
日本語の概要は準備中です。原文の説明を表示しています。
Compute genome-to-genome distances (ANI, AAI, dDDH, k-mer Mash) and assign taxonomic classifications using skani (Shaw 2023), FastANI (Jain 2018), pyani / pyANI ANIb / ANIm, OrthoANI (Lee 2016), AAI (amino-acid identity), dDDH via TYGS / GGDC, GTDB-Tk (Chaumeil 2020 standard prokaryote taxonomy), and Mash MinHash (Ondov 2016). Use when delineating prokaryote species (95% ANI threshold; Jain 2018 Nat Commun 9:5114), assigning genomes to GTDB taxonomy with ANI radius, computing genome similarity matrices for clustering, classifying archaea, evaluating MAG (metagenome-assembled genome) species assignment, applying skani for fast metagenomic ANI screening, or reconciling 16S rRNA-based taxonomy with whole-genome ANI.
日本語の概要は準備中です。原文の説明を表示しています。
Compute genome-to-genome distances (ANI, AAI, dDDH, k-mer Mash) and assign taxonomic classifications using skani (Shaw 2023), FastANI (Jain 2018), pyani / pyANI ANIb / ANIm, OrthoANI (Lee 2016), AAI (amino-acid identity), dDDH via TYGS / GGDC, GTDB-Tk (Chaumeil 2020 standard prokaryote taxonomy), and Mash MinHash (Ondov 2016). Use when delineating prokaryote species (95% ANI threshold; Jain 2018 Nat Commun 9:5114), assigning genomes to GTDB taxonomy with ANI radius, computing genome similarity matrices for clustering, classifying archaea, evaluating MAG (metagenome-assembled genome) species assignment, applying skani for fast metagenomic ANI screening, or reconciling 16S rRNA-based taxonomy with whole-genome ANI.
日本語の概要は準備中です。原文の説明を表示しています。
Analyzes, manipulates, compares, annotates, and visualizes phylogenetic or other hierarchical trees with ETE 4. Supports Newick/Nexus tree I/O, topology edits and pattern matching, Robinson-Foulds comparisons, gene-tree evolutionary events and reconciliation, NCBI/GTDB taxonomy, SmartView exploration, and publication rendering. Applies to existing trees after alignment and phylogenetic inference, rather than inferring trees from raw sequences.
日本語の概要は準備中です。原文の説明を表示しています。
Taxonomic classification of ASVs using reference databases like SILVA, GTDB, or UNITE. Covers naive Bayes classifiers (DADA2, IDTAXA) and exact matching approaches. Use when assigning taxonomy to ASVs after DADA2 amplicon processing.
日本語の概要は準備中です。原文の説明を表示しています。
Transforms vague prompts or feature requests into precise, testable specifications using EARS (Easy Approach to Requirements Syntax) grounded in relevant domain theories (GTD, BJ Fogg, Gestalt). Use when requirements lack triggers or measurable outcomes, or the user asks to "optimize my prompt" / "improve this requirement" / "make this more specific".
日本語の概要は準備中です。原文の説明を表示しています。
Assembles microbial-community sequencing into metagenome-assembled genomes (MAGs) with metaFlye (ONT), metaSPAdes/MEGAHIT (Illumina), and hifiasm-meta/metaMDBG (PacBio HiFi), then recovers genomes via multi-binner consolidation (MetaBAT2, MaxBin2, CONCOCT, SemiBin2, VAMB -> DAS_Tool) and QCs them against MIMAG with CheckM2, GUNC, and GTDB-Tk. Covers why a metagenome is not a genome (uneven coverage, micro-diversity, strain collapse to consensus), differential-coverage binning, co-assembly vs per-sample, the rRNA-operon collapse that fails short-read MAGs, and strain resolution with inStrain. Use when reconstructing genomes from a microbiome, soil, ocean, or gut community, recovering MAGs, or resolving strain-level variation.
日本語の概要は準備中です。原文の説明を表示しています。
Detects and removes contamination in genome assemblies via two disjoint workflows - foreign-sequence screening of a single-organism (eukaryote/isolate) assembly with NCBI FCS-GX (GenBank-submission-mandatory), FCS-adaptor, and BlobToolKit blob plots; and MAG/bin quality assessment with CheckM2 plus GUNC (chimerism) plus GTDB-Tk taxonomy, judged against MIMAG. Covers why CheckM2 alone is blind to disjoint-marker chimeras, the FCS-GX RAM wall, organelle/NUMT triage, strain heterogeneity, and the HGT-vs-contamination (tardigrade) trap. Use when screening an assembly for foreign contamination before GenBank submission, assessing MAG completeness/contamination/chimerism, deciding which contigs to remove, or distinguishing real HGT from contaminant contigs.
日本語の概要は準備中です。原文の説明を表示しています。
Synthesize a week of inputs (calendar, tasks, journal, OKR check-ins) into a structured review with wins, learnings, blockers, and next-week priorities. Use for a Friday/Sunday weekly review, GTD review, or OKR check-in.
日本語の概要は準備中です。原文の説明を表示しています。
Build a trusted capture-and-triage front door so no commitment lives in your head. Use when open loops keep slipping, an inbox has become a backlog, or vague notes never turn into action.
日本語の概要は準備中です。原文の説明を表示しています。
Detect contamination and assess genome quality using CheckM, CheckM2, GTDB-Tk, and GUNC for metagenome-assembled genomes and isolate assemblies. Use when checking assemblies for contamination.
日本語の概要は準備中です。原文の説明を表示しています。
Think and work like an expert Microbiologist. Use when a task calls for Microbiologist judgment. Reasons from culturability limits, CFU/MPN enumeration, selective media, DADA2/QIIME2 16S ASVs (SILVA/GTDB), and shotgun metagenomics (Kraken2, MetaPhlAn, HUMAnN); treats plate-count anomaly, compositional stats pitfalls, kit contamination, and index hopping as first-class failure modes.
日本語の概要は準備中です。原文の説明を表示しています。
Think and work like an expert Microbial Ecologist. Use when a task calls for Microbial Ecologist judgment. Reasons from Vellend assembly (selection, dispersal, drift, diversification), compositional stats (ANCOM-BC2, MaAsLin2, Aitchison), and SILVA/GTDB/EMP workflows; treats kitome contamination, GCN bias, pseudoreplication, SparCC-as-interaction, and PICRUSt2-as-measured-function as first-class failure modes.
日本語の概要は準備中です。原文の説明を表示しています。
Think and work like an expert Environmental Microbiologist. Use when a task calls for Environmental Microbiologist judgment. Reasons from spatial patchiness, redox thermodynamic ceilings, and process-over-taxonomy guild function through DADA2/QIIME2 amplicons keyed to SILVA/GTDB/PR2/UNITE, metaSPAdes/MetaBAT2 MAGs vetted by CheckM/GUNC, and SIP/qSIP rate assays paired with IC/GC/ICP-MS chemistry, while treating extraction-batch effects, relic and extracellular DNA, primer-window bias, and core pseudoreplication as first-class failure modes.
日本語の概要は準備中です。原文の説明を表示しています。
AI ผู้จัดการเวลา — Pomodoro, Time Block, GTD, Deep Work, Calendar Design สำหรับผู้ที่อยากควบคุมเวลาได้จริง
日本語の概要は準備中です。原文の説明を表示しています。
Assembles microbial-community sequencing into metagenome-assembled genomes (MAGs) with metaFlye (ONT), metaSPAdes/MEGAHIT (Illumina), and hifiasm-meta/metaMDBG (PacBio HiFi), then recovers genomes via multi-binner consolidation (MetaBAT2, MaxBin2, CONCOCT, SemiBin2, VAMB -> DAS_Tool) and QCs them against MIMAG with CheckM2, GUNC, and GTDB-Tk. Covers why a metagenome is not a genome (uneven coverage, micro-diversity, strain collapse to consensus), differential-coverage binning, co-assembly vs per-sample, the rRNA-operon collapse that fails short-read MAGs, and strain resolution with inStrain. Use when reconstructing genomes from a microbiome, soil, ocean, or gut community, recovering MAGs, or resolving strain-level variation.
日本語の概要は準備中です。原文の説明を表示しています。
Detects and removes contamination in genome assemblies via two disjoint workflows - foreign-sequence screening of a single-organism (eukaryote/isolate) assembly with NCBI FCS-GX (GenBank-submission-mandatory), FCS-adaptor, and BlobToolKit blob plots; and MAG/bin quality assessment with CheckM2 plus GUNC (chimerism) plus GTDB-Tk taxonomy, judged against MIMAG. Covers why CheckM2 alone is blind to disjoint-marker chimeras, the FCS-GX RAM wall, organelle/NUMT triage, strain heterogeneity, and the HGT-vs-contamination (tardigrade) trap. Use when screening an assembly for foreign contamination before GenBank submission, assessing MAG completeness/contamination/chimerism, deciding which contigs to remove, or distinguishing real HGT from contaminant contigs.
日本語の概要は準備中です。原文の説明を表示しています。